Urokinase type plasminogen activator and its receptor regulate the invasive potential of gastric cancer cell lines.

  • Authors:
    • Y Okusa
    • T Ichikura
    • H Mochizuki
    • N Shinomiya
  • View Affiliations

  • Published online on: November 1, 2000     https://doi.org/10.3892/ijo.17.5.1001
  • Pages: 1001-1006
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Abstract

To assess the role of urokinase-type plasminogen activator (uPA) and uPA receptor (uPAR) on the invasive potential of cancer cells, in vitro experiments were performed using two human gastric cancer cell lines, NUGC-3 and MKN-28. NUGC-3 cells secreted a higher level of uPA than MKN-28 cells, while the uPAR expression of NUGC-3 cells was lower than that of MKN-28 cells. Both cancer cell lines expressed Met protein and did not express hepatocyte growth factor (HGF). In Matrigel invasion assay, MKN-28 cells demonstrated significantly lower invasion index than NUGC-3 cells. The addition of exogenous uPA significantly increased the invasive activity of MKN-28 cells. The uPA expression in NUGC-3 cells was enhanced by adding conditioned media of fibroblast cells or HGF. These results suggest that uPA promotes the invasive capacity of the uPAR-positive cancer cells, and that stromal cells may play an important role in cancer cell invasion by supplying uPA and/or promoting uPA production.

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Nov 2000
Volume 17 Issue 5

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Okusa Y, Ichikura T, Mochizuki H and Shinomiya N: Urokinase type plasminogen activator and its receptor regulate the invasive potential of gastric cancer cell lines.. Int J Oncol 17: 1001-1006, 2000
APA
Okusa, Y., Ichikura, T., Mochizuki, H., & Shinomiya, N. (2000). Urokinase type plasminogen activator and its receptor regulate the invasive potential of gastric cancer cell lines.. International Journal of Oncology, 17, 1001-1006. https://doi.org/10.3892/ijo.17.5.1001
MLA
Okusa, Y., Ichikura, T., Mochizuki, H., Shinomiya, N."Urokinase type plasminogen activator and its receptor regulate the invasive potential of gastric cancer cell lines.". International Journal of Oncology 17.5 (2000): 1001-1006.
Chicago
Okusa, Y., Ichikura, T., Mochizuki, H., Shinomiya, N."Urokinase type plasminogen activator and its receptor regulate the invasive potential of gastric cancer cell lines.". International Journal of Oncology 17, no. 5 (2000): 1001-1006. https://doi.org/10.3892/ijo.17.5.1001