The affinity of MCF7 breast cancer cells to hyaluronan substrates of different molecular weight and concentrations in an in vitro model.

  • Authors:
    • G Egger
    • R Pfragner
    • V Siegl
    • K Zatloukal
    • A Glasner
    • A Bader
    • P Steindorfer
  • View Affiliations

  • Published online on: November 1, 2000     https://doi.org/10.3892/ijo.17.5.1019
  • Pages: 1019-1042
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Abstract

The affinity of MCF7 breast cancer cells to hyaluronan (HA) was investigated in an in vitro model. The cells form a tightly adhering monolayer on native HA with a concentration of 5 mg/ml. On native HA at higher concentrations the cells reduce their adhesion to the substrate in favor of increased intercellular bonds, resulting in a cluster-like aggregate that tends to detach from the substrate. Aggregate formation is accomplished after 12 h incubation. The phenomenon is independent of the CD44 receptor. Degradation of native HA by hyaluronidase abolishes aggregate formation even at high HA concentrations in favor of formation of a firmly adhering monolayer. This model may help to understand tumor spread on HA tissue structures and may explain therapy successes with hyaluronidase in tumor patients.

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Nov 2000
Volume 17 Issue 5

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Egger G, Pfragner R, Siegl V, Zatloukal K, Glasner A, Bader A and Steindorfer P: The affinity of MCF7 breast cancer cells to hyaluronan substrates of different molecular weight and concentrations in an in vitro model.. Int J Oncol 17: 1019-1042, 2000
APA
Egger, G., Pfragner, R., Siegl, V., Zatloukal, K., Glasner, A., Bader, A., & Steindorfer, P. (2000). The affinity of MCF7 breast cancer cells to hyaluronan substrates of different molecular weight and concentrations in an in vitro model.. International Journal of Oncology, 17, 1019-1042. https://doi.org/10.3892/ijo.17.5.1019
MLA
Egger, G., Pfragner, R., Siegl, V., Zatloukal, K., Glasner, A., Bader, A., Steindorfer, P."The affinity of MCF7 breast cancer cells to hyaluronan substrates of different molecular weight and concentrations in an in vitro model.". International Journal of Oncology 17.5 (2000): 1019-1042.
Chicago
Egger, G., Pfragner, R., Siegl, V., Zatloukal, K., Glasner, A., Bader, A., Steindorfer, P."The affinity of MCF7 breast cancer cells to hyaluronan substrates of different molecular weight and concentrations in an in vitro model.". International Journal of Oncology 17, no. 5 (2000): 1019-1042. https://doi.org/10.3892/ijo.17.5.1019