Gene expression abnormalities in human glial tumors identified by gene array

  • Authors:
    • Julia Y. Ljubimova
    • Natasha M. Khazenzon
    • Zutang Chen
    • Yulia I. Neyman
    • Laure Turner
    • Mary S. Riedinger
    • Keith L. Black
  • View Affiliations

  • Published online on: February 1, 2001     https://doi.org/10.3892/ijo.18.2.287
  • Pages: 287-295
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Abstract

Novel genes specific for human oligodendroglioma and glioblastoma multiforme (GBM) were detected using the gene array analysis [18,376 genes Gene Discovery Array (GDA) from Incyte Genomics, Inc.]. Eleven genes were chosen based on the highest ratios of differential expression identified by GDA between histologically normal adjacent tissue and brain tumor tissue. The differential expression of those 11 genes was verified by semiquantitative RT-PCR and Northern analysis on 22 samples of glial and other tumors of the brain, as well as of normal embryonic and adult brain tissue. Gene no. 5 (an EST) was more expressed by GDA analysis in histologically normal adjacent brain tissue than in the corresponding oligodendroglioma. By RT-PCR, this gene was expressed in a number of brain tumors but not in normal adult and embryonic brain. By GDA analysis, gene no. 7 (oligophrenin-1) gave the highest ratio compared to other genes in brain tissue adjacent to the GBM vs. GBM. By RT-PCR, oligophrenin-1 was expressed in tumors and tumor-adjacent tissue, whereas meningioma and corpus callosum were negative. Gene no. 11 (an EST) was expressed only in brain tumors but not in normal brain by Northern analysis (message size 1.5 kb) and RT-PCR. GDA analysis successfully identified genes preferentially expressed in brain tumors, which was confirmed by Northern analysis and semiquantitative RT-PCR. The validity of gene arrays for tumor-specific gene discovery is discussed. Study of differential gene expression in glial tumors should help identify the mechanism/s of transformation of normal glial cells to malignant.

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February 2001
Volume 18 Issue 2

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Ljubimova JY, Khazenzon NM, Chen Z, Neyman YI, Turner L, Riedinger MS and Black KL: Gene expression abnormalities in human glial tumors identified by gene array. Int J Oncol 18: 287-295, 2001.
APA
Ljubimova, J.Y., Khazenzon, N.M., Chen, Z., Neyman, Y.I., Turner, L., Riedinger, M.S., & Black, K.L. (2001). Gene expression abnormalities in human glial tumors identified by gene array. International Journal of Oncology, 18, 287-295. https://doi.org/10.3892/ijo.18.2.287
MLA
Ljubimova, J. Y., Khazenzon, N. M., Chen, Z., Neyman, Y. I., Turner, L., Riedinger, M. S., Black, K. L."Gene expression abnormalities in human glial tumors identified by gene array". International Journal of Oncology 18.2 (2001): 287-295.
Chicago
Ljubimova, J. Y., Khazenzon, N. M., Chen, Z., Neyman, Y. I., Turner, L., Riedinger, M. S., Black, K. L."Gene expression abnormalities in human glial tumors identified by gene array". International Journal of Oncology 18, no. 2 (2001): 287-295. https://doi.org/10.3892/ijo.18.2.287