Resistance of human leukemic cell lines to 1-β-D-arabinofuranosylcytosine: characterization of an experimental model

  • Authors:
    • Marzia B. Gariboldi
    • Raffaella Ravizza
    • Emanuela Marras
    • Gianpaolo Perletti
    • Alessandra De Simone
    • Francesco Piccinini
    • Elena Monti
  • View Affiliations

  • Published online on: June 1, 2001     https://doi.org/10.3892/ijo.18.6.1245
  • Pages: 1245-1249
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Abstract

1-β-D-arabinofuranosylcytosine (ara-C) is an antimetabolite used for the treatment of acute myelogenous leukemia. The ability of ara-C to kill neoplastic cells has been correlated to the induction of apoptosis. The clinical use of ara-C is limited by the development of drug resistance. Alterations in drug-induced apoptosis play a critical role in ara-C resistance. In particular, the proto-oncogene bcl-2 has been implicated in this phenomenon. To better understand the molecular basis of the role of bcl-2 in ara-C resistance, we investigated the relationship between the cytotoxic effect of ara-C, the expression levels and the subcellular localization of bcl-2 in three human leukemic cell lines (HL-60, KG1, J111). We have also evaluated the effects of ara-C on the J111 leukemic cell line (showing the lowest levels of Bcl-2 and the highest sensitivity to ara-C) overexpressing the bcl-2 oncogene. The model we developed here will allow further studies on the role of post-translational events involving bcl-2 (such as translocation and/or phosphorylation) in the cellular response to ara-C treatment.

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June 2001
Volume 18 Issue 6

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Gariboldi MB, Ravizza R, Marras E, Perletti G, De Simone A, Piccinini F and Monti E: Resistance of human leukemic cell lines to 1-β-D-arabinofuranosylcytosine: characterization of an experimental model. Int J Oncol 18: 1245-1249, 2001.
APA
Gariboldi, M.B., Ravizza, R., Marras, E., Perletti, G., De Simone, A., Piccinini, F., & Monti, E. (2001). Resistance of human leukemic cell lines to 1-β-D-arabinofuranosylcytosine: characterization of an experimental model. International Journal of Oncology, 18, 1245-1249. https://doi.org/10.3892/ijo.18.6.1245
MLA
Gariboldi, M. B., Ravizza, R., Marras, E., Perletti, G., De Simone, A., Piccinini, F., Monti, E."Resistance of human leukemic cell lines to 1-β-D-arabinofuranosylcytosine: characterization of an experimental model". International Journal of Oncology 18.6 (2001): 1245-1249.
Chicago
Gariboldi, M. B., Ravizza, R., Marras, E., Perletti, G., De Simone, A., Piccinini, F., Monti, E."Resistance of human leukemic cell lines to 1-β-D-arabinofuranosylcytosine: characterization of an experimental model". International Journal of Oncology 18, no. 6 (2001): 1245-1249. https://doi.org/10.3892/ijo.18.6.1245