S-adenosylmethionine decarboxylase overexpression reduces invasiveness and tumorigenicity in nude mice of MCF-7 breast cancer cells

  • Authors:
    • Andrea Manni
    • Stephen Fischer
    • Matthew Franks
    • Sharlene Washington
    • Rena De Arment
    • James Griffith
    • Laurence Demers
    • Michael Verderame
    • Benjamin Leiby
    • David Mauger
  • View Affiliations

  • Published online on: August 1, 2001     https://doi.org/10.3892/ijo.19.2.317
  • Pages: 317-323
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Abstract

To elucidate the role of S-adenosylmethionine decarboxylase (SAMDC) in breast cancer biology, we have generated SAMDC overexpressing MCF-7 breast cancer cells. SAMDC overexpression did not alter in a major way growth properties of MCF-7 cells in soft agar, either under basal conditions or in response to estrogen and antiestrogen administration. SAMDC-MCF-7 cells, on the other hand, exhibited a markedly reduced invasive ability in matrigel (p=0.013). Furthermore, they were less tumorigenic in nude mice. The odds for control clones to form tumors were 3.13 (C.1.1.2-8.2, p=0.0184) higher than those for SAMDC clones. The odds ratio were identical in the absence and in the presence of estradiol. In addition, the growth rate of established tumors was slower for SAMDC than for control clones. Overall, our results are consistent with the notion that these phenotypic changes induced by SAMDC overexpression are primarily mediated by suppression of cellular putrescine (and, possibly, spermidine) levels.

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August 2001
Volume 19 Issue 2

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Manni A, Fischer S, Franks M, Washington S, De Arment R, Griffith J, Demers L, Verderame M, Leiby B, Mauger D, Mauger D, et al: S-adenosylmethionine decarboxylase overexpression reduces invasiveness and tumorigenicity in nude mice of MCF-7 breast cancer cells. Int J Oncol 19: 317-323, 2001
APA
Manni, A., Fischer, S., Franks, M., Washington, S., De Arment, R., Griffith, J. ... Mauger, D. (2001). S-adenosylmethionine decarboxylase overexpression reduces invasiveness and tumorigenicity in nude mice of MCF-7 breast cancer cells. International Journal of Oncology, 19, 317-323. https://doi.org/10.3892/ijo.19.2.317
MLA
Manni, A., Fischer, S., Franks, M., Washington, S., De Arment, R., Griffith, J., Demers, L., Verderame, M., Leiby, B., Mauger, D."S-adenosylmethionine decarboxylase overexpression reduces invasiveness and tumorigenicity in nude mice of MCF-7 breast cancer cells". International Journal of Oncology 19.2 (2001): 317-323.
Chicago
Manni, A., Fischer, S., Franks, M., Washington, S., De Arment, R., Griffith, J., Demers, L., Verderame, M., Leiby, B., Mauger, D."S-adenosylmethionine decarboxylase overexpression reduces invasiveness and tumorigenicity in nude mice of MCF-7 breast cancer cells". International Journal of Oncology 19, no. 2 (2001): 317-323. https://doi.org/10.3892/ijo.19.2.317