Involvement of PI3K in PKCε-mediated oncogenic signal in rat colonic epithelial cells

  • Authors:
    • Emanuela Marras
    • Paola Concari
    • Lara Cortellezzi
    • Davide Dondi
    • Magda De Eguileor
    • Gianpaolo Perletti
  • View Affiliations

  • Published online on: August 1, 2001     https://doi.org/10.3892/ijo.19.2.395
  • Pages: 395-399
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Abstract

We have recently demonstrated that overexpression of PKCε is oncogenic in colonic epithelial cells. To test whether PI3K might be an upstream effector of PKCε in cell transformation, we have overexpressed the p110α PI3K subunit in non-transformed D/WT colonic epithelial cells. Transfectants displayed the major in vitro features of transformed cells. Interestingly, no transformation occurred when p110α was co-transfected with a dead-kinase PKCε mutant. The p85α subunit of PI3K, displaying a dominant-negative-like effect, was then transfected in PKCε-transformed D/ε cells. The transformed profile of these cells was markedly reduced. To identify which by-products of PI3K might be involved in cell transformation we have transfected the D/WT cell line with cDNAs encoding the PI3 kinases hVps34 and C2β. Overexpression of hVps34 did not cause cell transformation. Conversely, in vitro transformation was observed when C2β was transfected into D/WT cells. These results indicate that phosphatidylinositol-3 monophosphate does not seem to be involved in cell transformation, and that phosphatidylinositol-3,4 bisphosphate and phosphatidylinositol-3,4,5 trisphosphate are more likely involved in this process. Thus, our data support the hypothesis of a linkage between PI3K and PKCε, and indicate that PI3K may act as a source of second messengers responsible for oncogenic activation of PKCε.

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August 2001
Volume 19 Issue 2

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Marras E, Concari P, Cortellezzi L, Dondi D, De Eguileor M and Perletti G: Involvement of PI3K in PKCε-mediated oncogenic signal in rat colonic epithelial cells. Int J Oncol 19: 395-399, 2001
APA
Marras, E., Concari, P., Cortellezzi, L., Dondi, D., De Eguileor, M., & Perletti, G. (2001). Involvement of PI3K in PKCε-mediated oncogenic signal in rat colonic epithelial cells. International Journal of Oncology, 19, 395-399. https://doi.org/10.3892/ijo.19.2.395
MLA
Marras, E., Concari, P., Cortellezzi, L., Dondi, D., De Eguileor, M., Perletti, G."Involvement of PI3K in PKCε-mediated oncogenic signal in rat colonic epithelial cells". International Journal of Oncology 19.2 (2001): 395-399.
Chicago
Marras, E., Concari, P., Cortellezzi, L., Dondi, D., De Eguileor, M., Perletti, G."Involvement of PI3K in PKCε-mediated oncogenic signal in rat colonic epithelial cells". International Journal of Oncology 19, no. 2 (2001): 395-399. https://doi.org/10.3892/ijo.19.2.395