LACK OF MUTATION AT CODON 12 OF THE C-H-RAS ONCOGENE IN 51 HUMAN SARCOMAS ASSESSED BY PCR-RFLP

  • Authors:
    • JS CASTRESANA
    • MP RUBIO
    • K KUNIMI
    • C BARRIOS
    • A KREICBERGS
  • View Affiliations

  • Published online on: May 1, 1993     https://doi.org/10.3892/ijo.2.5.823
  • Pages: 823-825
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Abstract

Mutations of the ras genes in codons 12, 13 or 61 make them oncogenic. There are several methods to determine the point mutations in the ras family. In an attempt to do so in a sensitive and time saving manner we have checked a total of 51 sarcomas (14 bone tumors and 37 soft tissue tumors) for mutations in codon 12 of the c-H-ras oncogene. First we amplified a 100 bp fragment covering this codon and then performed RFLP analysis after digestion with restriction endonuclease Msp I. We did not find mutations in any of the tumors. Our results, may reinforce previous data on the fact that ras genes do not play an important role in tumorigenesis of sarcomas.

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May 1993
Volume 2 Issue 5

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
CASTRESANA J, RUBIO M, KUNIMI K, BARRIOS C and KREICBERGS A: LACK OF MUTATION AT CODON 12 OF THE C-H-RAS ONCOGENE IN 51 HUMAN SARCOMAS ASSESSED BY PCR-RFLP. Int J Oncol 2: 823-825, 1993.
APA
CASTRESANA, J., RUBIO, M., KUNIMI, K., BARRIOS, C., & KREICBERGS, A. (1993). LACK OF MUTATION AT CODON 12 OF THE C-H-RAS ONCOGENE IN 51 HUMAN SARCOMAS ASSESSED BY PCR-RFLP. International Journal of Oncology, 2, 823-825. https://doi.org/10.3892/ijo.2.5.823
MLA
CASTRESANA, J., RUBIO, M., KUNIMI, K., BARRIOS, C., KREICBERGS, A."LACK OF MUTATION AT CODON 12 OF THE C-H-RAS ONCOGENE IN 51 HUMAN SARCOMAS ASSESSED BY PCR-RFLP". International Journal of Oncology 2.5 (1993): 823-825.
Chicago
CASTRESANA, J., RUBIO, M., KUNIMI, K., BARRIOS, C., KREICBERGS, A."LACK OF MUTATION AT CODON 12 OF THE C-H-RAS ONCOGENE IN 51 HUMAN SARCOMAS ASSESSED BY PCR-RFLP". International Journal of Oncology 2, no. 5 (1993): 823-825. https://doi.org/10.3892/ijo.2.5.823