INFLUENCE OF INSULIN-LIKE GROWTH FACTOR-I ANTISERUM TO PROLIFERATION OF T-CELL RECEPTOR-ACTIVATED HUMAN T-LYMPHOCYTES
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- Published online on: June 1, 1993 https://doi.org/10.3892/ijo.2.6.1003
- Pages: 1003-1008
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Abstract
We have previously reported that Insulin-like growth factor (IGF-I) is a cellular growth factor of human transformed T-lymphocytes. To evaluate the role of IGF-I for nontransformed T-cells we investigated synthesis of IGF-I and the influence of an IGF-I antiserum, UBK487, during proliferation process. IGF-I is synthesized by B-lymphocytes and monocytes during proliferation. Via the T-cell receptor (CD3 subunit) induced proliferation of resting T-cells was inhibited by addition of UBK487. The addition of monocytes to the resting T-cell fraction reversed the UBK487 effect. Using UBK487, a 12 kDa protein could be immunoprecipitated from unstimulated or PHA stimulated T-cells whereas a 36 kDa protein could be immunoprecipitated from OKT3 stimulated T-cells.
