Cellular sensitivity determinants to docetaxel in human gastrointestinal cancers

Park,J.-S.; Yamamoto,W.; Sekikawa,T.; Matsukawa,M.; Okamoto,R.; Sasaki,M.; Ukon,K.; Tanimoto,K.; Kumazaki,T.; Nishiyama,M.

doi:10.3892/ijo.20.2.333

Cellular sensitivity determinants to docetaxel in human gastrointestinal cancers

Authors:
- J.-S. Park
- W. Yamamoto
- T. Sekikawa
- M. Matsukawa
- R. Okamoto
- M. Sasaki
- K. Ukon
- K. Tanimoto
- T. Kumazaki
- M. Nishiyama
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Affiliations: Department of Biochemistry and Biophysics, Research Institute for Radiation Biology and Medicine, Hiroshima University, Hiroshima, Japan
Published online on: February 1, 2002 https://doi.org/10.3892/ijo.20.2.333
Pages: 333-338

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Abstract

β-tubulin (β-TUB), Bcl-XL, and additionally glutathione S-transferase π (GSTπ) were found to participate in sensitivity to docetaxel (TXT) in 7 human gastrointestinal cancer cell lines. The gene expression level of β-TUB, Bcl-XL, and GSTπ was closely correlated with the IC50 for TXT. β-TUB amount related to TXT resistance, and GST activity was correlated with IC50 for TXT in the 30-min treatment setting. Bcl-XL transfection increased TXT resistance of COLO201 cells, whereas GST inhibition by ethacrynic acid enhanced TXT cytotoxicity. Continuous TXT treatment increased β-TUB and GSTπ expression, but the increased GSTπ mRNA was observed in TXT-resistant HCC-48 cells alone.