Genome wide detection of oncogene amplifications in nasopharyngeal carcinoma by array based comparative genomic hybridization

  • Authors:
    • Angela Bik-Yu Hui
    • Kwok-Wai Lo
    • Peter M.L. Teo
    • Ka-Fai To
    • Dolly P. Huang
  • View Affiliations

  • Published online on: March 1, 2002     https://doi.org/10.3892/ijo.20.3.467
  • Pages: 467-473
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Abstract

We have applied the method of genomic microarray to investigate amplification of oncogenes throughout the genome of nasopharyngeal carcinoma (NPC). Array based comparative genomic hybridization (array CGH) allows simultaneous examination of 58 oncogenes commonly amplified in various human cancers. In the present study, we have examined 15 NPC samples including five cell lines, two xenografts and eight primary tumours with array CGH to reveal the particular oncogenes associated with this cancer. This is the first genome wide survey of multiple oncogene amplifications involved in the development of NPC. Non-random gene amplifications were identified for the first time in NPC on MYCL1 in 1p34.3 and on TERC and PIK3CA at 3q26.3. Other high level amplified oncogenes included NRAS, RAF1, MYB, EGFR, FGF4, EMS1, and D17S167. Highest frequencies of gain of novel oncogenes were detected on MYCL1 (66.7%), TERC (46.7%), ESR (46.7%), PIK3CA (40%), LAMC2 (33.3%), and CSE1L (33.3%).

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March 2002
Volume 20 Issue 3

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Hui AB, Lo K, Teo PM, To K and Huang DP: Genome wide detection of oncogene amplifications in nasopharyngeal carcinoma by array based comparative genomic hybridization. Int J Oncol 20: 467-473, 2002
APA
Hui, A.B., Lo, K., Teo, P.M., To, K., & Huang, D.P. (2002). Genome wide detection of oncogene amplifications in nasopharyngeal carcinoma by array based comparative genomic hybridization. International Journal of Oncology, 20, 467-473. https://doi.org/10.3892/ijo.20.3.467
MLA
Hui, A. B., Lo, K., Teo, P. M., To, K., Huang, D. P."Genome wide detection of oncogene amplifications in nasopharyngeal carcinoma by array based comparative genomic hybridization". International Journal of Oncology 20.3 (2002): 467-473.
Chicago
Hui, A. B., Lo, K., Teo, P. M., To, K., Huang, D. P."Genome wide detection of oncogene amplifications in nasopharyngeal carcinoma by array based comparative genomic hybridization". International Journal of Oncology 20, no. 3 (2002): 467-473. https://doi.org/10.3892/ijo.20.3.467