A rapid screening assay for antioxidant potential of natural and synthetic agents in vitro

  • Authors:
    • Praveen Srinivasan
    • Manicka V. Vadhanam
    • Jamal M. Arif
    • Ramesh C. Gupta
  • View Affiliations

  • Published online on: May 1, 2002     https://doi.org/10.3892/ijo.20.5.983
  • Pages: 983-986
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Abstract

There are literally thousands of known agents with potential chemopreventive and antioxidant activity; however, the expanding list of natural and synthetic compounds makes it difficult to test every agent in the widely accepted 2-year animal bioassay and human clinical trials. Therefore, short-term screening assays are needed to sort out the most efficacious compounds for long-term animal studies. In the present study, the identification of chemopreventive agents with efficacious antioxidant potential was explored with a Cu2+-mediated Fenton-type reaction, coupled with oxidative DNA lesion detection by 32P-postlabeling. Several agents inhibited the formation of 8-oxo-2'-deoxyguanosine (8-oxodG), a benchmark oxidative DNA lesion, but ellagic acid, a polyphenol found in berries, offered maximal (>80%) inhibition of 8-oxodG formation. However, a well-known tea polyphenol, epigallocatechin gallate, along with silymarin and D,L-sulforaphane, exhibited a pro-oxidant effect, with 50-70% increase in 8-oxodG induction. In general, our results agree with the reported antioxidant - pro-oxidant activities of the compounds, rendering this in vitro screening assay to be useful in determining the antioxidant potential of compounds rapidly and cost-effectively.

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May 2002
Volume 20 Issue 5

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Srinivasan P, Vadhanam MV, Arif JM and Gupta RC: A rapid screening assay for antioxidant potential of natural and synthetic agents in vitro. Int J Oncol 20: 983-986, 2002.
APA
Srinivasan, P., Vadhanam, M.V., Arif, J.M., & Gupta, R.C. (2002). A rapid screening assay for antioxidant potential of natural and synthetic agents in vitro. International Journal of Oncology, 20, 983-986. https://doi.org/10.3892/ijo.20.5.983
MLA
Srinivasan, P., Vadhanam, M. V., Arif, J. M., Gupta, R. C."A rapid screening assay for antioxidant potential of natural and synthetic agents in vitro". International Journal of Oncology 20.5 (2002): 983-986.
Chicago
Srinivasan, P., Vadhanam, M. V., Arif, J. M., Gupta, R. C."A rapid screening assay for antioxidant potential of natural and synthetic agents in vitro". International Journal of Oncology 20, no. 5 (2002): 983-986. https://doi.org/10.3892/ijo.20.5.983