Identifying cancer-related genes in nasopharyngeal carcinoma cell lines using DNA and mRNA expression profiling analyses

  • Authors:
    • Xiang Guo
    • Weng-Onn Lui
    • Chao-Nan Qian
    • Jin-Dong Chen
    • Steven George Gray
    • Daniel Rhodes
    • Brian Haab
    • Eric Stanbridge
    • Huimin Wang
    • Ming-Huang Hong
    • Hua-Qing Min
    • Catharina Larsson
    • Bin Tean Teh
  • View Affiliations

  • Published online on: December 1, 2002     https://doi.org/10.3892/ijo.21.6.1197
  • Pages: 1197-1204
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Abstract

The goals of this study were to evaluate the potential of detecting cryptic amplification and deletion of cancer-related genes using array-based comparative genomic hybridization (CGH), and to identify candidate cancer genes by combined parallel analyses of copy number and gene expression profiles in nasopharyngeal carcinoma (NPC) cell lines. We established global DNA copy number and mRNA expression profiles on human NPC cell lines using a high-density cDNA microarray. The DNA copy number alterations detected by array CGH were compared to the DNA copy number variations identified by metaphase CGH. A cryptic amplification at 3q26 was detected by array CGH, which was not found by metaphase CGH. By amplicon mapping and parallel analyses of DNA copy number and mRNA expression levels, we identified several candidates which could be important mediators in tumor formation or progression. Taken together, the combination of copy number and gene expression profiling using cDNA microarrays provides an improved strategy for gene discovery in human cancer.

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December 2002
Volume 21 Issue 6

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Copy and paste a formatted citation
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Spandidos Publications style
Guo X, Lui W, Qian C, Chen J, Gray SG, Rhodes D, Haab B, Stanbridge E, Wang H, Hong M, Hong M, et al: Identifying cancer-related genes in nasopharyngeal carcinoma cell lines using DNA and mRNA expression profiling analyses. Int J Oncol 21: 1197-1204, 2002.
APA
Guo, X., Lui, W., Qian, C., Chen, J., Gray, S.G., Rhodes, D. ... Teh, B.T. (2002). Identifying cancer-related genes in nasopharyngeal carcinoma cell lines using DNA and mRNA expression profiling analyses. International Journal of Oncology, 21, 1197-1204. https://doi.org/10.3892/ijo.21.6.1197
MLA
Guo, X., Lui, W., Qian, C., Chen, J., Gray, S. G., Rhodes, D., Haab, B., Stanbridge, E., Wang, H., Hong, M., Min, H., Larsson, C., Teh, B. T."Identifying cancer-related genes in nasopharyngeal carcinoma cell lines using DNA and mRNA expression profiling analyses". International Journal of Oncology 21.6 (2002): 1197-1204.
Chicago
Guo, X., Lui, W., Qian, C., Chen, J., Gray, S. G., Rhodes, D., Haab, B., Stanbridge, E., Wang, H., Hong, M., Min, H., Larsson, C., Teh, B. T."Identifying cancer-related genes in nasopharyngeal carcinoma cell lines using DNA and mRNA expression profiling analyses". International Journal of Oncology 21, no. 6 (2002): 1197-1204. https://doi.org/10.3892/ijo.21.6.1197