Phosphorylation of p53, induction of Bax and activation of caspases during β-lapachone-mediated apoptosis in human prostate epithelial cells

  • Authors:
    • Yung Hyun Choi
    • Mi Jung Kim
    • Seung Yeon Lee
    • Youl-Nam Lee
    • Gyoo Yong Chi
    • Hyun Sup Eom
    • Nam Deuk Kim
    • Byung Tae Choi
  • View Affiliations

  • Published online on: December 1, 2002     https://doi.org/10.3892/ijo.21.6.1293
  • Pages: 1293-1299
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Abstract

The DNA topoisomerase I inhibitor β-lapachone, the product of a tree from South America, is known to exhibit various biological properties, the mechanisms of which are poorly understood. We investigated the effects of β-lapachone on the growth of human prostate epithelial cells. Upon treatment with β-lapachone, a concentration-dependent inhibition of cell viability was observed and cells developed many of the hallmark features of apoptosis, including condensation of chromatin and DNA fragmentation. The apoptotic effects of β-lapachone were associated with marked induction of p53 phosphorylation and Bax protein without altering the expression of p53 and Bcl-2 protein. In addition, the proteolytic cleavage of specific target proteins such as poly(ADP-ribose) polymerase, β-catenin and Rad51, which are hallmarks of apoptosis, were observed, and Western blotting demonstrated that processing/activation of caspases release cytochrome c from the mitochondria into the cytosol and accompany the generation of β-lapachone-mediating apoptotic cell death. However, β-lapachone did not affect the levels of c-IAP family proteins. The present results suggest that apoptotic signals evoked by β-lapachone in human prostate epithelial cells may converge caspases activation through up-regulation of phosphorylation of p53 and Bax rather than down-regulation of c-IAPs family.

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December 2002
Volume 21 Issue 6

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Choi YH, Kim MJ, Lee SY, Lee Y, Chi GY, Eom HS, Kim ND and Choi BT: Phosphorylation of p53, induction of Bax and activation of caspases during β-lapachone-mediated apoptosis in human prostate epithelial cells. Int J Oncol 21: 1293-1299, 2002
APA
Choi, Y.H., Kim, M.J., Lee, S.Y., Lee, Y., Chi, G.Y., Eom, H.S. ... Choi, B.T. (2002). Phosphorylation of p53, induction of Bax and activation of caspases during β-lapachone-mediated apoptosis in human prostate epithelial cells. International Journal of Oncology, 21, 1293-1299. https://doi.org/10.3892/ijo.21.6.1293
MLA
Choi, Y. H., Kim, M. J., Lee, S. Y., Lee, Y., Chi, G. Y., Eom, H. S., Kim, N. D., Choi, B. T."Phosphorylation of p53, induction of Bax and activation of caspases during β-lapachone-mediated apoptosis in human prostate epithelial cells". International Journal of Oncology 21.6 (2002): 1293-1299.
Chicago
Choi, Y. H., Kim, M. J., Lee, S. Y., Lee, Y., Chi, G. Y., Eom, H. S., Kim, N. D., Choi, B. T."Phosphorylation of p53, induction of Bax and activation of caspases during β-lapachone-mediated apoptosis in human prostate epithelial cells". International Journal of Oncology 21, no. 6 (2002): 1293-1299. https://doi.org/10.3892/ijo.21.6.1293