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Isolation and characterisation of vascular endothelial growth factor-165 specific scFv fragments by phage display

  • Authors:
    • K. A. Smith
    • N. Kirkpatrick
    • L. A. Madden
    • K. P. Topping
    • J. R.T. Monson
    • J. Greenman

  • View Affiliations

  • Published online on: February 1, 2003     https://doi.org/10.3892/ijo.22.2.333
  • Pages: 333-338
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Abstract

Vascular endothelial growth factor (VEGF) is a multifunctional cytokine which plays a major role in angiogenesis. Alternative splicing causes the production of several different isoforms (VEGF-A 121, 145, 165, 189, 206). VEGF is essential for tumor angiogenesis and several studies have correlated elevated VEGF levels with tumor stage, metastases and progression. Antibody phage display was employed to isolate two scFv antibody fragments, D8 and F10, with specificity for the VEGF165 isoform. It was shown by ELISA and competitive immunohistochemistry that each clone bound to VEGF165 but not VEGF121. Immunohistochemistry with D8 and F10 on colorectal carcinoma and adenoma sections revealed positive staining similar to that shown by a polyclonal VEGF antibody. The scFv antibody fragments, D8 and F10, will be useful in specifically detecting circulating VEGF165 in cancer patients as most studies to date have quantified the total level of circulating VEGF (121 and 165). These reagents will allow further elucidation of the role of VEGF in tumor angiogenesis.

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February 2003
Volume 22 Issue 2

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Smith KA, Kirkpatrick N, Madden LA, Topping KP, Monson JR and Greenman J: Isolation and characterisation of vascular endothelial growth factor-165 specific scFv fragments by phage display. Int J Oncol 22: 333-338, 2003
APA
Smith, K.A., Kirkpatrick, N., Madden, L.A., Topping, K.P., Monson, J.R., & Greenman, J. (2003). Isolation and characterisation of vascular endothelial growth factor-165 specific scFv fragments by phage display. International Journal of Oncology, 22, 333-338. https://doi.org/10.3892/ijo.22.2.333
MLA
Smith, K. A., Kirkpatrick, N., Madden, L. A., Topping, K. P., Monson, J. R., Greenman, J."Isolation and characterisation of vascular endothelial growth factor-165 specific scFv fragments by phage display". International Journal of Oncology 22.2 (2003): 333-338.
Chicago
Smith, K. A., Kirkpatrick, N., Madden, L. A., Topping, K. P., Monson, J. R., Greenman, J."Isolation and characterisation of vascular endothelial growth factor-165 specific scFv fragments by phage display". International Journal of Oncology 22, no. 2 (2003): 333-338. https://doi.org/10.3892/ijo.22.2.333