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Reversal of drug resistance mediated by hammerhead ribozyme against multidrug resistance-associated protein 1 in a human glioma cell line

  • Authors:
    • Hideo Osada
    • Tetsuji Tokunaga
    • Yoshiyuki Abe
    • Satomi Asai
    • Hayato Miyachi
    • Hiroyuki Hatanaka
    • Atsushi Tsugu
    • Hiroshi Kijima
    • Hitoshi Yamazaki
    • Katsuji Shima
    • Yoshito Ueyama
    • Yoshiyuki Osamura
    • Masato Nakamura

  • View Affiliations

  • Published online on: April 1, 2003     https://doi.org/10.3892/ijo.22.4.823
  • Pages: 823-827
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Abstract

We examined the effects of suppressing multidrug resistance-associated protein 1 (MRP1) gene expression in a human glioma cell line U87MG. Hammerhead ribozymes, designed to cleave MRP1 mRNA (αMRP1-Rz), were transfected into the U87MG cells. The U87MG/αMRP1-Rz cells were significantly sensitive to nitrosourea (ACNU) and doxorubicin (DOX) compared with the U87MG cells (p<0.01 and p<0.05, respectively, unpaired t-test). There was no significant difference in the expression of other human genes between the U87MG/αMRP1-Rz and controls by cDNA array. The hammerhead ribozyme-mediated specific suppression of MRP1 was sufficient to reverse the resistance of ACNU and DOX in the human glioma cell line.

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Journal Cover

April 2003
Volume 22 Issue 4

Print ISSN: 1019-6439
Online ISSN:1791-2423

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  • Journal Metrics
  • Impact Factor: 5.2
  • Ranked Oncology
    (total number of cites)
  • CiteScore: 11.4
  • CiteScore Rank: Q1: #40/366 Oncology
  • Source Normalized Impact per Paper (SNIP): 1.125
  • SCImago Journal Rank (SJR): 1.105
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Spandidos Publications style
Osada H, Tokunaga T, Abe Y, Asai S, Miyachi H, Hatanaka H, Tsugu A, Kijima H, Yamazaki H, Shima K, Shima K, et al: Reversal of drug resistance mediated by hammerhead ribozyme against multidrug resistance-associated protein 1 in a human glioma cell line. Int J Oncol 22: 823-827, 2003
APA
Osada, H., Tokunaga, T., Abe, Y., Asai, S., Miyachi, H., Hatanaka, H. ... Nakamura, M. (2003). Reversal of drug resistance mediated by hammerhead ribozyme against multidrug resistance-associated protein 1 in a human glioma cell line. International Journal of Oncology, 22, 823-827. https://doi.org/10.3892/ijo.22.4.823
MLA
Osada, H., Tokunaga, T., Abe, Y., Asai, S., Miyachi, H., Hatanaka, H., Tsugu, A., Kijima, H., Yamazaki, H., Shima, K., Ueyama, Y., Osamura, Y., Nakamura, M."Reversal of drug resistance mediated by hammerhead ribozyme against multidrug resistance-associated protein 1 in a human glioma cell line". International Journal of Oncology 22.4 (2003): 823-827.
Chicago
Osada, H., Tokunaga, T., Abe, Y., Asai, S., Miyachi, H., Hatanaka, H., Tsugu, A., Kijima, H., Yamazaki, H., Shima, K., Ueyama, Y., Osamura, Y., Nakamura, M."Reversal of drug resistance mediated by hammerhead ribozyme against multidrug resistance-associated protein 1 in a human glioma cell line". International Journal of Oncology 22, no. 4 (2003): 823-827. https://doi.org/10.3892/ijo.22.4.823