A novel neoplastic primary tumor-derived human prostate epithelial cell line

  • Authors:
    • Daejin Ko
    • Yongpeng Gu
    • Yutaka Yasunaga
    • Keiichiro Nakamura
    • Shiv Srivastava
    • Judd W. Moul
    • Isabell A. Sesterhenn
    • David G. McLeod
    • Paul Arnstein
    • D. O. Taylor
    • Bharati Hukku
    • Johng S. Rhim
  • View Affiliations

  • Published online on: June 1, 2003     https://doi.org/10.3892/ijo.22.6.1311
  • Pages: 1311-1317
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Research into molecular and genetic mechanisms underlying prostate carcinogenesis would be greatly advanced by in vitro models of prostate tumors representing primary tumors. The generation of immortalized primary prostate cancer cells that will accurately reflect the in situ characteristics of malignant epithelium is greatly needed. We have successfully established a neoplastic immortalized human prostate epithelial (HPE) cell culture derived from a primary tumor. The RC-9 cells transduced through infection with a retrovirus vector expressing the E6 and E7 genes (E6E7) of human papilloma virus-16 (HPV-16) are currently growing well at passage 40, whereas RC-9 cells senesced at passage 7. RC-9/E6E7 cells exhibit epithelial morphology and high level of telomerase activity. More importantly, these immortalized cells produced tumors (SCID5038D) when inoculated into SCID mice. RC-9/E6E7 cells and SCID-5038D cells exhibit a high level of telomerase activity and androgen-responsiveness when treated with R1881. Expression of prostate specific antigen (PSA), androgen receptor (AR), prostate stem cell antigen (PSCA), an androgen-regulated prostate specific gene (NKX3.1), p16, cytokeratins 8, 15 and HPV-16 E6 gene was detected in both of these cells. RC-9/E6E7 and SCID5038D cells also showed growth inhibition when exposed to retinoic acid and transforming growth factor (TGF)-β1, potent inhibitors of prostate epithelial cell growth. A number of chromosome alterations were observed including the loss of chromosomes 2p, 3p, 8p, 13, 14, 16, 17, 18, 21 and the gain of 7 and 20 in the tumor cell line (SCID5038D). These results demonstrate that this primary tumor-derived HPE cell line retained its neoplastic phenotypes and its prostate-specific markers and should allow studies to elucidate molecular and genetic alterations involved in prostate cancer. This is the first documented case of a malignant AR and PSA positive established human prostate cancer cell line from a primary tumor of a prostate cancer patient.

Related Articles

Journal Cover

June 2003
Volume 22 Issue 6

Print ISSN: 1019-6439
Online ISSN:1791-2423

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Ko D, Gu Y, Yasunaga Y, Nakamura K, Srivastava S, Moul JW, Sesterhenn IA, McLeod DG, Arnstein P, Taylor DO, Taylor DO, et al: A novel neoplastic primary tumor-derived human prostate epithelial cell line. Int J Oncol 22: 1311-1317, 2003
APA
Ko, D., Gu, Y., Yasunaga, Y., Nakamura, K., Srivastava, S., Moul, J.W. ... Rhim, J.S. (2003). A novel neoplastic primary tumor-derived human prostate epithelial cell line. International Journal of Oncology, 22, 1311-1317. https://doi.org/10.3892/ijo.22.6.1311
MLA
Ko, D., Gu, Y., Yasunaga, Y., Nakamura, K., Srivastava, S., Moul, J. W., Sesterhenn, I. A., McLeod, D. G., Arnstein, P., Taylor, D. O., Hukku, B., Rhim, J. S."A novel neoplastic primary tumor-derived human prostate epithelial cell line". International Journal of Oncology 22.6 (2003): 1311-1317.
Chicago
Ko, D., Gu, Y., Yasunaga, Y., Nakamura, K., Srivastava, S., Moul, J. W., Sesterhenn, I. A., McLeod, D. G., Arnstein, P., Taylor, D. O., Hukku, B., Rhim, J. S."A novel neoplastic primary tumor-derived human prostate epithelial cell line". International Journal of Oncology 22, no. 6 (2003): 1311-1317. https://doi.org/10.3892/ijo.22.6.1311