Sodium salicylate induces apoptosis in HCT116 colorectal cancer cells through activation of p38MAPK

  • Authors:
    • Eun Ju Lee
    • Hye Geong Park
    • Ho Sung Kang
  • View Affiliations

  • Published online on: August 1, 2003     https://doi.org/10.3892/ijo.23.2.503
  • Pages: 503-508
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Sodium salicylate is known to induce apoptosis in a variety of cancer cells. However, the molecular mechanism for salicylate-induced apoptosis is yet unclear. Here we show that in HCT116 colon carcinoma cells, 10 mM sodium salicylate induces caspase-3 activation and degradation of its substrates, poly(ADP-ribose) polymerase (PARP), β-catenin, and retinoblastoma (Rb). In contrast, sodium salicylate did not exert any significant effects on the expression of Fas L that is implicated in extrinsic apoptotic pathway and the levels of Bcl-2 family proteins, Bcl-2, Bcl-xsl, and Bad, which are involved in intrinsic apoptotic pathway, and anti-apoptotic molecules, c-IAP1 and HSP73. In addition, 10 mM salicylate induced p53 tumor suppressor protein that plays an important role in cell cycle arrest or apoptosis and the induction seemed to be linked to its phosphorylation at Set 15. To investigate the signal pathways for salicylate-induced apoptosis, we examined the effects of sodium salicylate on protein kinase activities. Sodium salicylate activated p38MAPK through phosphorylation at Thr 180/Tyr 182 and Akt/PKB at Ser 473, whereas it partially activated ERK1/2 through its phosphorylation at Thr 202/Tyr 204. We also show that SB203580 (a specific p38MAPK inhibitor), but not other protein kinase inhibitors (PD98059, LY294002, and wortmannin), significantly prevented salicylate-induced apoptosis. These results suggest that sodium salicylate-induced apoptosis in HCT116 colorectal cancer cells is mediated by p38MAPK.

Related Articles

Journal Cover

August 2003
Volume 23 Issue 2

Print ISSN: 1019-6439
Online ISSN:1791-2423

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Lee EJ, Park HG and Kang HS: Sodium salicylate induces apoptosis in HCT116 colorectal cancer cells through activation of p38MAPK. Int J Oncol 23: 503-508, 2003.
APA
Lee, E.J., Park, H.G., & Kang, H.S. (2003). Sodium salicylate induces apoptosis in HCT116 colorectal cancer cells through activation of p38MAPK. International Journal of Oncology, 23, 503-508. https://doi.org/10.3892/ijo.23.2.503
MLA
Lee, E. J., Park, H. G., Kang, H. S."Sodium salicylate induces apoptosis in HCT116 colorectal cancer cells through activation of p38MAPK". International Journal of Oncology 23.2 (2003): 503-508.
Chicago
Lee, E. J., Park, H. G., Kang, H. S."Sodium salicylate induces apoptosis in HCT116 colorectal cancer cells through activation of p38MAPK". International Journal of Oncology 23, no. 2 (2003): 503-508. https://doi.org/10.3892/ijo.23.2.503