Targeting foreign major histocompatibility complex molecules to tumors by tumor cell specific single chain antibody (scFv)

  • Authors:
    • Jinhua Li
    • Karl J. Franek
    • Andrea L. Patterson
    • Lillia M. Holmes
    • Kelly. E. Burgin
    • Jianfei Ji
    • Xianzhong Yu
    • Thomas E. Wagner
    • Yanzhang Wei
  • View Affiliations

  • Published online on: November 1, 2003     https://doi.org/10.3892/ijo.23.5.1329
  • Pages: 1329-1332
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Abstract

Down-regulation of the major histocompatibility complex (MHC) is one of the major mechanisms that tumor cells adopted to escape immunosurveillance. Therefore, specifically coating tumor cells with foreign MHC may make tumor cells a better target for immune recognition and surveillance. In this study, we designed and generated a fusion protein, H2Kd/scPSMA, consisting of a single chain antibody against human prostate specific membrane antigen (PSMA) and the extracellular domain of mouse H-2Kd. The expression of this fusion protein in B16F0 mouse melanoma cells was confirmed by RT-PCR and fluorescent activated cell sorting (FACS). Our animal study showed that the expression of H2Kd/scPSMA in B16F0/PSMA5, a B16F0 cell line expressing human PSMA, significantly inhibited tumor growth as demonstrated in the pulmonary metastasis assay and tumor growth study and improved overall survival.

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November 2003
Volume 23 Issue 5

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Li J, Franek KJ, Patterson AL, Holmes LM, Burgin KE, Ji J, Yu X, Wagner TE and Wei Y: Targeting foreign major histocompatibility complex molecules to tumors by tumor cell specific single chain antibody (scFv). Int J Oncol 23: 1329-1332, 2003.
APA
Li, J., Franek, K.J., Patterson, A.L., Holmes, L.M., Burgin, K.E., Ji, J. ... Wei, Y. (2003). Targeting foreign major histocompatibility complex molecules to tumors by tumor cell specific single chain antibody (scFv). International Journal of Oncology, 23, 1329-1332. https://doi.org/10.3892/ijo.23.5.1329
MLA
Li, J., Franek, K. J., Patterson, A. L., Holmes, L. M., Burgin, K. E., Ji, J., Yu, X., Wagner, T. E., Wei, Y."Targeting foreign major histocompatibility complex molecules to tumors by tumor cell specific single chain antibody (scFv)". International Journal of Oncology 23.5 (2003): 1329-1332.
Chicago
Li, J., Franek, K. J., Patterson, A. L., Holmes, L. M., Burgin, K. E., Ji, J., Yu, X., Wagner, T. E., Wei, Y."Targeting foreign major histocompatibility complex molecules to tumors by tumor cell specific single chain antibody (scFv)". International Journal of Oncology 23, no. 5 (2003): 1329-1332. https://doi.org/10.3892/ijo.23.5.1329