HER2 testing in gastric cancer: Molecular morphology and storage time-related changes in archival samples

  • Authors:
    • Mauro Risio
    • Giovanni De Rosa
    • Ivana Sarotto
    • Laura Casorzo
    • Lorenzo Capussotti
    • Bruno Torchio
    • Massimo Aglietta
    • Laura Chiecchio
  • View Affiliations

  • Published online on: November 1, 2003     https://doi.org/10.3892/ijo.23.5.1381
  • Pages: 1381-1387
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Abstract

Seventy-two archival specimens of advanced gastric adenocarcinoma were analyzed by fluorescence in situ hybridization and immunohistochemistry for HER2 gene status. Gene amplification and concomitant protein overexpression were detected in 15.2% of cases. Intratumor topographical variability of HER2 amplification was observed, indicative of the late involvement of HER gene in gastric tumorigenesis. No significant correlations were found between HER2 status and histopathologic type, grade of differentiation, and Goseki classification of carcinomas, suggesting that HER2 gene is variably linked with the morphogenesis of gastric adenocarcinoma. A significant decrease of the incidence of HER2 amplified carcinomas in function of the duration of the storage of paraffin blocks was observed: 42-33% for tumor specimens paraffin-embedded in 2002-2001; 8.3%, 15.7%, 11.1% for the years 2000, 1999, 1998, respectively; 0% for cases embedded during 1997. According to these results, the reliability of the FISH and immunohistochemical assays decreases after prolonged storage of paraffin-embedded specimens.

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November 2003
Volume 23 Issue 5

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Risio M, De Rosa G, Sarotto I, Casorzo L, Capussotti L, Torchio B, Aglietta M and Chiecchio L: HER2 testing in gastric cancer: Molecular morphology and storage time-related changes in archival samples. Int J Oncol 23: 1381-1387, 2003
APA
Risio, M., De Rosa, G., Sarotto, I., Casorzo, L., Capussotti, L., Torchio, B. ... Chiecchio, L. (2003). HER2 testing in gastric cancer: Molecular morphology and storage time-related changes in archival samples. International Journal of Oncology, 23, 1381-1387. https://doi.org/10.3892/ijo.23.5.1381
MLA
Risio, M., De Rosa, G., Sarotto, I., Casorzo, L., Capussotti, L., Torchio, B., Aglietta, M., Chiecchio, L."HER2 testing in gastric cancer: Molecular morphology and storage time-related changes in archival samples". International Journal of Oncology 23.5 (2003): 1381-1387.
Chicago
Risio, M., De Rosa, G., Sarotto, I., Casorzo, L., Capussotti, L., Torchio, B., Aglietta, M., Chiecchio, L."HER2 testing in gastric cancer: Molecular morphology and storage time-related changes in archival samples". International Journal of Oncology 23, no. 5 (2003): 1381-1387. https://doi.org/10.3892/ijo.23.5.1381