Cyclin A correlates with the sensitivity of human cancer cells to cytotoxic effects of 5-FU

  • Authors:
    • Ryuichi Kawashima
    • Minoru Haisa
    • Masashi Kimura
    • Munenori Takaoka
    • Yasuhiro Shirakawa
    • Haruo Takeda
    • Hirokazu Uetsuka
    • Mehmet Gunduz
    • Noriyuki Nagai
    • Noriaki Tanaka
    • Yoshio Naomoto
  • View Affiliations

  • Published online on: February 1, 2004     https://doi.org/10.3892/ijo.24.2.273
  • Pages: 273-278
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Abstract

We investigated the role of cyclin A in the cytotoxic effect of 5-fluorouracil (5-FU) on cancer cell lines. Experiments were performed using gastric cancer chemosensitive NUGC3, and chemoresistant NUGC3/5FU/L established by repeated exposure to 5-FU. 5-FU inhibited cell growth of NUGC3 in a dose-dependent manner. Low concentrations of 5-FU did not inhibit cell growth of NUGC3/5FU/L, while high concentrations slightly inhibited cell growth. Examination of the cell cycle pattern of NUGC3 cells showed accumulation at S-phase at 10 µM and at G1-S-phase at 100 µM of 5-FU. Cell cycle pattern of NUGC3/5FU/L cells did not change 5-FU concentrations. 5-FU increased cyclin A mRNA level in NUGC3 cells but not NUGC3/5FU/L cells. In the presence of 100 µM 5-FU, cyclin A protein level increased 2.6-fold in NUGC3 and 1.47-fold in NUGC3/5FU/L. 5-FU dose-dependently increased the percentage of cyclin A-positive NUGC3 cells, but not NUGC3/5FU/L cells. The percentage of cyclin A-positive cells in other 5-FU sensitive esophageal, colon and gastric cancer cell lines (T.Tn, LOVO, DLD-1, MKN-7), increased in the presence of 1 and 10 µM 5-FU, while cyclin A-positive cells in 5-FU resitant hepatocellular and colon carcinoma cell lines (HCC50 and C-1), did not increase with the same treatment. Our results indicate that the cytotoxic effects of 5-FU in human cancer cell lines correlate with cyclin A and it may be used as a predictive factor for chemotherapy response.

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February 2004
Volume 24 Issue 2

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Kawashima R, Haisa M, Kimura M, Takaoka M, Shirakawa Y, Takeda H, Uetsuka H, Gunduz M, Nagai N, Tanaka N, Tanaka N, et al: Cyclin A correlates with the sensitivity of human cancer cells to cytotoxic effects of 5-FU. Int J Oncol 24: 273-278, 2004
APA
Kawashima, R., Haisa, M., Kimura, M., Takaoka, M., Shirakawa, Y., Takeda, H. ... Naomoto, Y. (2004). Cyclin A correlates with the sensitivity of human cancer cells to cytotoxic effects of 5-FU. International Journal of Oncology, 24, 273-278. https://doi.org/10.3892/ijo.24.2.273
MLA
Kawashima, R., Haisa, M., Kimura, M., Takaoka, M., Shirakawa, Y., Takeda, H., Uetsuka, H., Gunduz, M., Nagai, N., Tanaka, N., Naomoto, Y."Cyclin A correlates with the sensitivity of human cancer cells to cytotoxic effects of 5-FU". International Journal of Oncology 24.2 (2004): 273-278.
Chicago
Kawashima, R., Haisa, M., Kimura, M., Takaoka, M., Shirakawa, Y., Takeda, H., Uetsuka, H., Gunduz, M., Nagai, N., Tanaka, N., Naomoto, Y."Cyclin A correlates with the sensitivity of human cancer cells to cytotoxic effects of 5-FU". International Journal of Oncology 24, no. 2 (2004): 273-278. https://doi.org/10.3892/ijo.24.2.273