Interleukin-1β and tumour necrosis factor-α promote the transformation of human immortalised mesothelial cells by erionite

  • Authors:
    • Yaohe Wang
    • Steven P. Faux
    • Gunnel Hallden
    • David H. Kirn
    • Cathy E. Houghton
    • Nicholas R. Lemoine
    • Graham Patrick
  • View Affiliations

  • Published online on: July 1, 2004     https://doi.org/10.3892/ijo.25.1.173
  • Pages: 173-178
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Abstract

Asbestos fails to induce the transformation of human mesothelial cells in vitro although it has been known as a potential carcinogen to human mesothelial cells. Interleukin-1β (IL-1β) and tumour necrosis factor-α (TNF-α) are major cytokines released by macrophages after inhalation of asbestos. These cytokines can regulate mesothelial cell proliferation both in vitro and in vivo. In the present study, we used the growth in soft agar as an index of transformation and investigated the role of IL-1β and TNF-α during the process of human mesothelial cell carcinogenesis. Both IL-1β and TNF-α were demonstrated to enhance erionite-induced transformation of the immortalised, non-tumorigenic human mesothelial cell line (MeT-5A) in vitro. The MeT-5A cells could only be transformed when the cells were exposed to a combination of cytokines and erionite, or at least two cytokines together without erionite, for at least 4 months in vitro. The findings presented here suggest that IL-1β and TNF-α play a significant role in the pathogenesis of mesothelioma, and that it might be desirable to block or inhibit cytokine secretion in high risk populations to prevent mesothelioma.

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July 2004
Volume 25 Issue 1

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Wang Y, Faux SP, Hallden G, Kirn DH, Houghton CE, Lemoine NR and Patrick G: Interleukin-1β and tumour necrosis factor-α promote the transformation of human immortalised mesothelial cells by erionite. Int J Oncol 25: 173-178, 2004.
APA
Wang, Y., Faux, S.P., Hallden, G., Kirn, D.H., Houghton, C.E., Lemoine, N.R., & Patrick, G. (2004). Interleukin-1β and tumour necrosis factor-α promote the transformation of human immortalised mesothelial cells by erionite. International Journal of Oncology, 25, 173-178. https://doi.org/10.3892/ijo.25.1.173
MLA
Wang, Y., Faux, S. P., Hallden, G., Kirn, D. H., Houghton, C. E., Lemoine, N. R., Patrick, G."Interleukin-1β and tumour necrosis factor-α promote the transformation of human immortalised mesothelial cells by erionite". International Journal of Oncology 25.1 (2004): 173-178.
Chicago
Wang, Y., Faux, S. P., Hallden, G., Kirn, D. H., Houghton, C. E., Lemoine, N. R., Patrick, G."Interleukin-1β and tumour necrosis factor-α promote the transformation of human immortalised mesothelial cells by erionite". International Journal of Oncology 25, no. 1 (2004): 173-178. https://doi.org/10.3892/ijo.25.1.173