Diagnostic and classification value of metalloproteinases in squamous human laryngeal carcinoma

  • Authors:
    • T. A. Christopoulos
    • N. Papageorgakopoulou
    • D. A. Theocharis
    • A. J. Aletras
    • C. P. Tsiganos
    • T. A. Papadas
    • N. S. Mastronikolis
    • P. Goumas
    • D. H. Vynios
  • View Affiliations

  • Published online on: August 1, 2004     https://doi.org/10.3892/ijo.25.2.481
  • Pages: 481-485
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Abstract

Metalloproteinases (MMPs) are a class of enzymes largely involved in tumour progression and metastasis. At least twenty different enzymes are recognized that are also present under normal state of tissues. Their activity is regulated by their presence as proenzymes and by the concomitant presence of the respective tissue inhibitors (TIMPs). The present study describes the alterations of MMPs observed in human laryngeal carcinoma with respect to tumour classification and compares their activity in normal and cancerous tissues and biopsy specimens. Samples from five patients who underwent laryngectomy, from five biopsies and three from autopsies were used. The MMPs of normal and malignant human laryngeal cartilage and of biopsy specimens were identified immunochemically and by zymography using gelatin or casein as substrates. Healthy cartilage from autopsies was found to contain almost exclusively MMP-1, proMMP-2 and proMMP-9. Normal parts from laryngectomies contained, in addition, significant amounts of active MMP-2. The respective malignant parts contained both MMP-2 and -9 in increased amounts in their latent and active forms. Similar profile of MMPs was also identified in tissues surrounding affected cartilage. These alterations were found to be in good accordance with tumour stage and were also observed in biopsy samples. Thus, analysis of MMPs in biopsies can be used together with the clinicopathological parameters for the classification or early diagnosis of laryngeal tumours.

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August 2004
Volume 25 Issue 2

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Christopoulos TA, Papageorgakopoulou N, Theocharis DA, Aletras AJ, Tsiganos CP, Papadas TA, Mastronikolis NS, Goumas P and Vynios DH: Diagnostic and classification value of metalloproteinases in squamous human laryngeal carcinoma. Int J Oncol 25: 481-485, 2004
APA
Christopoulos, T.A., Papageorgakopoulou, N., Theocharis, D.A., Aletras, A.J., Tsiganos, C.P., Papadas, T.A. ... Vynios, D.H. (2004). Diagnostic and classification value of metalloproteinases in squamous human laryngeal carcinoma. International Journal of Oncology, 25, 481-485. https://doi.org/10.3892/ijo.25.2.481
MLA
Christopoulos, T. A., Papageorgakopoulou, N., Theocharis, D. A., Aletras, A. J., Tsiganos, C. P., Papadas, T. A., Mastronikolis, N. S., Goumas, P., Vynios, D. H."Diagnostic and classification value of metalloproteinases in squamous human laryngeal carcinoma". International Journal of Oncology 25.2 (2004): 481-485.
Chicago
Christopoulos, T. A., Papageorgakopoulou, N., Theocharis, D. A., Aletras, A. J., Tsiganos, C. P., Papadas, T. A., Mastronikolis, N. S., Goumas, P., Vynios, D. H."Diagnostic and classification value of metalloproteinases in squamous human laryngeal carcinoma". International Journal of Oncology 25, no. 2 (2004): 481-485. https://doi.org/10.3892/ijo.25.2.481