COX-2 correlates with F-box protein, Skp2 expression and prognosis in human gastric carcinoma

  • Authors:
    • Soichiro Honjo
    • Satoru Kase
    • Mitsuhiko Osaki
    • Tonang Dwi Ardyanto
    • Nobuaki Kaibara
    • Hisao Ito
  • View Affiliations

  • Published online on: February 1, 2005     https://doi.org/10.3892/ijo.26.2.353
  • Pages: 353-360
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

The expression of cyclooxygenase (COX)-2 is induced by growth factors, tumor promoters and cytokines, and is correlated with carcinogenesis, tumor progression and inhibition of apoptosis. To clarify the pathological significance of COX-2, we examined the effect of a selective COX-2 inhibitor, NS398, on two human gastric carcinoma cell lines, MKN-45 and KATO-III, and the expression of Skp2, P27/Kip1 and COX-2 protein in human gastric carcinomas. NS398 inhibited cell growth in a time- and dose-dependent manner and exerted cell cycle arrest in the G0/G1 phase without induction of apoptosis in MKN-45, but had no effect in KATO-III. In MKN-45, NS398 induced up-regulation of P27/Kip1 and down-regulation of COX-2, cyclin D1 and Skp2. Immunohistochemistry using 63 surgically resected gastric carcinomas disclosed that COX-2 expression was correlated with Skp2 expression and that P27/Kip1 expression was inversely correlated with COX-2 and Skp2 expression. High levels of COX-2 or Skp2 were significantly correlated with poor survival (P=0.02 and P=0.004). Our results suggested that: a) NS398 induced inhibition of cell proliferation through cell cycle arrest and suppressed the expression of Skp2 in COX-2-expressing gastric carcinoma cells, and b) COX-2 contributes to the expression of Skp2 and poor survival in human gastric carcinomas.

Related Articles

Journal Cover

February 2005
Volume 26 Issue 2

Print ISSN: 1019-6439
Online ISSN:1791-2423

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Honjo S, Kase S, Osaki M, Ardyanto TD, Kaibara N and Ito H: COX-2 correlates with F-box protein, Skp2 expression and prognosis in human gastric carcinoma. Int J Oncol 26: 353-360, 2005.
APA
Honjo, S., Kase, S., Osaki, M., Ardyanto, T.D., Kaibara, N., & Ito, H. (2005). COX-2 correlates with F-box protein, Skp2 expression and prognosis in human gastric carcinoma. International Journal of Oncology, 26, 353-360. https://doi.org/10.3892/ijo.26.2.353
MLA
Honjo, S., Kase, S., Osaki, M., Ardyanto, T. D., Kaibara, N., Ito, H."COX-2 correlates with F-box protein, Skp2 expression and prognosis in human gastric carcinoma". International Journal of Oncology 26.2 (2005): 353-360.
Chicago
Honjo, S., Kase, S., Osaki, M., Ardyanto, T. D., Kaibara, N., Ito, H."COX-2 correlates with F-box protein, Skp2 expression and prognosis in human gastric carcinoma". International Journal of Oncology 26, no. 2 (2005): 353-360. https://doi.org/10.3892/ijo.26.2.353