The zinc-finger transcription factor Snail downregulates proliferating cell nuclear antigen expression in colorectal carcinoma cells

  • Authors:
    • Jae-Hong Park
    • Ik-Joo Sung
    • Sae-Won Lee
    • Kyu-Won Kim
    • Young-Shin Kim
    • Mi-Ae Yoo
  • View Affiliations

  • Published online on: June 1, 2005     https://doi.org/10.3892/ijo.26.6.1541
  • Pages: 1541-1547
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Abstract

The Snail family of zinc-finger protein is a transcription repressor that is involved in the development of vertebrate and invertebrate embryos as well as in tumor progression. This family leads to broad biological functions such as cell differentiation, cell motility, cell cycle regulation and apoptosis. However, the target genes of Snail remain little known. In this study, we found several potential Snail binding sequences in the 5'-flanking region of human PCNA gene. Cotransfection experiments showed that Snail reduces human PCNA gene promoter activity in colorectal carcinoma cell lines, HCT116 and Colo320HSR. Snail-reduced PCNA expression was detected in immunoblotting and immunochemistry. In BrdU incorporation experiment, Snail inhibited the BrdU incorporation. Electrophoretic mobility shift assays showed that Snail can bind to the potential Snail recognition sites in the human PCNA promoter. Taken together, our results suggest that Snail may have an inhibitory effect on cell proliferation through down-regulation of PCNA expression as a novel target.

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June 2005
Volume 26 Issue 6

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Park J, Sung I, Lee S, Kim K, Kim Y and Yoo M: The zinc-finger transcription factor Snail downregulates proliferating cell nuclear antigen expression in colorectal carcinoma cells. Int J Oncol 26: 1541-1547, 2005.
APA
Park, J., Sung, I., Lee, S., Kim, K., Kim, Y., & Yoo, M. (2005). The zinc-finger transcription factor Snail downregulates proliferating cell nuclear antigen expression in colorectal carcinoma cells. International Journal of Oncology, 26, 1541-1547. https://doi.org/10.3892/ijo.26.6.1541
MLA
Park, J., Sung, I., Lee, S., Kim, K., Kim, Y., Yoo, M."The zinc-finger transcription factor Snail downregulates proliferating cell nuclear antigen expression in colorectal carcinoma cells". International Journal of Oncology 26.6 (2005): 1541-1547.
Chicago
Park, J., Sung, I., Lee, S., Kim, K., Kim, Y., Yoo, M."The zinc-finger transcription factor Snail downregulates proliferating cell nuclear antigen expression in colorectal carcinoma cells". International Journal of Oncology 26, no. 6 (2005): 1541-1547. https://doi.org/10.3892/ijo.26.6.1541