Adenoviral gene transduction of hepatocyte growth factor elicits inhibitory effects for hepatoma

  • Authors:
    • Kentaro Yuge
    • Tomoyuki Takahashi
    • Satoshi Nagano
    • Yasuhiro Terazaki
    • Yoshiteru Murofushi
    • Hiroaki Ushikoshi
    • Takao Kawai
    • Ngin Cin Khai
    • Toshikazu Nakamura
    • Hisayoshi Fujiwara
    • Ken-Ichiro Kosai
  • View Affiliations

  • Published online on: July 1, 2005     https://doi.org/10.3892/ijo.27.1.77
  • Pages: 77-85
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Abstract

Hepatocyte growth factor (HGF) gene therapy may have potential for treating chronic hepatitis (CH) and liver cirrhosis (LC). However, the lack of an HGF gene therapy study on hepatomas that are often associated with CH or LC, together with the stimulatory effects of HGF on many types of cancer, may hamper its application. This study explored the effects of adenoviral HGF gene transduction and their mechanisms on two types of hepatoma cells (hepatoblastoma and hepatocellular carcinoma) in in vitro experiments. Both types of hepatomas were revealed to have higher adenoviral gene transduction efficiencies and more efficient expressions of the HGF transgene, which successfully activated the HGF receptor/c-Met in an autocrine fashion, than those of other types of cancer. Notably, not only HGF, but also adenoviral infection, inhibited DNA synthesis, whereas only HGF but not adenoviral infection exerted a potent apoptotic effect. Moreover, adenoviral HGF gene transduction additively exerted inhibitory effects on cisplatin-treated hepatomas. In conclusion, inhibitory and apoptotic effects of adenoviral HGF gene transduction in hepatomas in contrast to potent mitogenic and antiapoptotic effects of HGF for hepatocytes are not only of biological interest, but also pose clinical benefits for adenoviral HGF gene therapy for CH and LC.

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July 2005
Volume 27 Issue 1

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Yuge K, Takahashi T, Nagano S, Terazaki Y, Murofushi Y, Ushikoshi H, Kawai T, Khai NC, Nakamura T, Fujiwara H, Fujiwara H, et al: Adenoviral gene transduction of hepatocyte growth factor elicits inhibitory effects for hepatoma. Int J Oncol 27: 77-85, 2005.
APA
Yuge, K., Takahashi, T., Nagano, S., Terazaki, Y., Murofushi, Y., Ushikoshi, H. ... Kosai, K. (2005). Adenoviral gene transduction of hepatocyte growth factor elicits inhibitory effects for hepatoma. International Journal of Oncology, 27, 77-85. https://doi.org/10.3892/ijo.27.1.77
MLA
Yuge, K., Takahashi, T., Nagano, S., Terazaki, Y., Murofushi, Y., Ushikoshi, H., Kawai, T., Khai, N. C., Nakamura, T., Fujiwara, H., Kosai, K."Adenoviral gene transduction of hepatocyte growth factor elicits inhibitory effects for hepatoma". International Journal of Oncology 27.1 (2005): 77-85.
Chicago
Yuge, K., Takahashi, T., Nagano, S., Terazaki, Y., Murofushi, Y., Ushikoshi, H., Kawai, T., Khai, N. C., Nakamura, T., Fujiwara, H., Kosai, K."Adenoviral gene transduction of hepatocyte growth factor elicits inhibitory effects for hepatoma". International Journal of Oncology 27, no. 1 (2005): 77-85. https://doi.org/10.3892/ijo.27.1.77