Growth inhibitory effect of triple anti-tumor gene transfer using Semliki Forest virus vector in glioblastoma cells

  • Authors:
    • Jong-Soo Lee
    • Jun-Han Lee
    • Haryoung Poo
    • Mi-Suk Kim
    • Seung-Hoon Lee
    • Moon-Hee Sung
    • Chul-Joong Kim
  • View Affiliations

  • Published online on: March 1, 2006     https://doi.org/10.3892/ijo.28.3.649
  • Pages: 649-654
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Abstract

The gene delivery of multiple tumor suppressors can provide an efficient tumor therapy in the case of malignant human glioblastomas containing multiple genetic alteration and inactivation. As such, the current study presents a new delivery system that can simultaneously express three anti-tumor genes using a Semliki Forest virus (SFV) vector in the expectation of combined or synergistic effects of angiogenesis inhibition by angiostatin and apoptosis induction by p53, PTEN and the rSFV particle itself. Recombinant SFV (rSFV) containing three anti-tumor genes (rSFV-Agt/p53/PTEN) were found to efficiently transduce and express each anti-tumor gene in glioblastoma cells. In addition, rSFV-Agt/p53/PTEN also resulted in a more effective induction of apoptosis in vitro and inhibition of tumor growth in nude mice when compared with other rSFVs containing only one or two anti-tumor genes. Accordingly, the current results demonstrate that a triple anti-tumor gene transfer using an rSFV vector would be a powerful strategy for regional cancer gene therapy.

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March 2006
Volume 28 Issue 3

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Lee J, Lee J, Poo H, Kim M, Lee S, Sung M and Kim C: Growth inhibitory effect of triple anti-tumor gene transfer using Semliki Forest virus vector in glioblastoma cells. Int J Oncol 28: 649-654, 2006
APA
Lee, J., Lee, J., Poo, H., Kim, M., Lee, S., Sung, M., & Kim, C. (2006). Growth inhibitory effect of triple anti-tumor gene transfer using Semliki Forest virus vector in glioblastoma cells. International Journal of Oncology, 28, 649-654. https://doi.org/10.3892/ijo.28.3.649
MLA
Lee, J., Lee, J., Poo, H., Kim, M., Lee, S., Sung, M., Kim, C."Growth inhibitory effect of triple anti-tumor gene transfer using Semliki Forest virus vector in glioblastoma cells". International Journal of Oncology 28.3 (2006): 649-654.
Chicago
Lee, J., Lee, J., Poo, H., Kim, M., Lee, S., Sung, M., Kim, C."Growth inhibitory effect of triple anti-tumor gene transfer using Semliki Forest virus vector in glioblastoma cells". International Journal of Oncology 28, no. 3 (2006): 649-654. https://doi.org/10.3892/ijo.28.3.649