Generation and characterization of monoclonal antibodies against choline kinase α and their potential use as diagnostic tools in cancer
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- Published online on: August 1, 2006 https://doi.org/10.3892/ijo.29.2.335
- Pages: 335-340
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Abstract
Choline kinase α (ChoKα) is a metabolic enzyme involved in the synthesis of phosphatidylcholine, recently implicated in cancer onset since it is overexpressed in a variety of human cancers such as mammary, lung, colorectal and prostate adenocarcinomas. Furthermore, overexpression of ChoKα in human HEK293T cells confers them oncogenic properties with the induction of tumors after subcutaneous injection in nude mice. ChoKα levels in tumor samples have been analyzed using polyclonal antibodies and Western blotting. These techniques have considerable limitations and do not allow for a precise and efficient evaluation of the real significance of ChoK overexpression in human carcinogenesis. We developed a set of monoclonal antibodies with high specificity and sensitivity against ChoKα, and characterized their properties. We provide evidence that the newly generated MoAbs against ChoKα have potential use in cancer diagnosis by conventional immunohistochemistry techniques.
