Membrane vesicles shed by oligodendroglioma cells induce neuronal apoptosis

  • Authors:
    • Stefania D'Agostino
    • Monica Salamone
    • Italia Di Liegro
    • M. Letizia Vittorelli
  • View Affiliations

  • Published online on: November 1, 2006     https://doi.org/10.3892/ijo.29.5.1075
  • Pages: 1075-1085
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

In order to investigate the mechanism by which oligodendrogliomas cause neuronal damage, media conditioned by G26/24 oligodendroglioma cells, were fractionated into shed vesicles and vesicle-free supernatants, and added to primary cultures of rat fetal cortical neurons. After one night treatment with vesicles, a reproducible, dose-dependent, inhibitory effect on neurite outgrowth was already induced and, after 48-72 h of incubation, neuronal apoptosis was evident. Vesicle-free supernatants and vesicles shed by NIH-3T3 cells had no inhibitory effects on neurons. Western blot analyses showed that treated neurons expressed a decreased amount of neurofilament (NF), growth-associated protein (GAP-43) and microtubule-associated protein (MAP-2). Moreover procaspase-3 and -8 were activated while Bcl-2 expression was reduced. Vesicles were found positive for the proapoptotic molecule, Fas-ligand (Fas-L), and for the B isoform of Nogo protein, a myelin component with inhibitory effects on neurons. Nogo B involvement in the vesicle effects was analyzed both by testing the neutralizing capability of anti-Nogo antibodies and by removing the Nogo receptor from neurons by phospholipase C digestion. These treatments did not revert the vesicle effects. To test the role of Fas-L, vesicles were treated with functional anti-Fas-L monoclonals. Vesicle inhibitory and proapoptotic effects were reduced. Vesicles shed by ovarian carcinoma cells (OvCa), which are known to vehicle biologically active Fas-L, had similar effects on neurons to those of oligodendroglioma vesicles, and their inhibitory effects were also reduced by anti Fas-L antibodies. We therefore conclude that vesicles shed by G26/24 cells induce neuronal apoptosis at least partially by a Fas-L mediated mechanism.

Related Articles

Journal Cover

November 2006
Volume 29 Issue 5

Print ISSN: 1019-6439
Online ISSN:1791-2423

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
D'Agostino S, Salamone M, Di Liegro I and Vittorelli ML: Membrane vesicles shed by oligodendroglioma cells induce neuronal apoptosis. Int J Oncol 29: 1075-1085, 2006
APA
D'Agostino, S., Salamone, M., Di Liegro, I., & Vittorelli, M.L. (2006). Membrane vesicles shed by oligodendroglioma cells induce neuronal apoptosis. International Journal of Oncology, 29, 1075-1085. https://doi.org/10.3892/ijo.29.5.1075
MLA
D'Agostino, S., Salamone, M., Di Liegro, I., Vittorelli, M. L."Membrane vesicles shed by oligodendroglioma cells induce neuronal apoptosis". International Journal of Oncology 29.5 (2006): 1075-1085.
Chicago
D'Agostino, S., Salamone, M., Di Liegro, I., Vittorelli, M. L."Membrane vesicles shed by oligodendroglioma cells induce neuronal apoptosis". International Journal of Oncology 29, no. 5 (2006): 1075-1085. https://doi.org/10.3892/ijo.29.5.1075