Elevated levels of tumour endothelial marker-8 in human breast cancer and its clinical significance

  • Authors:
    • Gaynor Davies
    • Khaled A. Rmali
    • Gareth Watkins
    • Robert E. Mansel
    • Malcolm D. Mason
    • Wen G. Jiang
  • View Affiliations

  • Published online on: November 1, 2006     https://doi.org/10.3892/ijo.29.5.1311
  • Pages: 1311-1317
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Abstract

Tumour endothelial marker-8 (TEM-8) belongs to a family of endothelial markers that are raised during tumour angiogenesis. We have recently reported aberrant expression of TEMs at the mRNA level in human breast cancer. This study sought to examine the level of TEM-8 expression at the protein and mRNA level in human breast cancer tissue, and in a panel of human breast cancer cell lines. We also wished to determine if TEM-8 can be used as a suitable marker for identifying tumour associated micro-vessels. At the mRNA level more tumours showed positive TEM-8 expression compared to normal background tissue. TEM-8 was detected in a variety of breast cancer cell lines, endothelial cells (HECV) and in a human fibroblast cell line (MRC5) at both the mRNA and protein level. Using immunohistochemistry the distribution of TEM-8 staining was more widespread in invasive breast cancer tissue compared to normal background tissue. Furthermore, the TEM-8 marker was found to be more discriminatory in identifying micro-vessels in tumour endothelium (2.8±0.83 vs. normal 1.66±0.52; P<0.011), compared to the vWFA marker (1.61±0.54 vs. normal 2.71±0.76; P<0.009). Raised levels of TEM-8 were associated with shorter survival outcome, but were not correlated to disease-free survival as shown by Kaplan-Meier and Cox regression analysis. We conclude that TEM-8 is a useful marker for identifying tumour associated micro-vessels and that elevated levels are associated with disease progression, which may have some bearing on the prognostic outcome in breast cancer.

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November 2006
Volume 29 Issue 5

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Davies G, Rmali KA, Watkins G, Mansel RE, Mason MD and Jiang WG: Elevated levels of tumour endothelial marker-8 in human breast cancer and its clinical significance. Int J Oncol 29: 1311-1317, 2006.
APA
Davies, G., Rmali, K.A., Watkins, G., Mansel, R.E., Mason, M.D., & Jiang, W.G. (2006). Elevated levels of tumour endothelial marker-8 in human breast cancer and its clinical significance. International Journal of Oncology, 29, 1311-1317. https://doi.org/10.3892/ijo.29.5.1311
MLA
Davies, G., Rmali, K. A., Watkins, G., Mansel, R. E., Mason, M. D., Jiang, W. G."Elevated levels of tumour endothelial marker-8 in human breast cancer and its clinical significance". International Journal of Oncology 29.5 (2006): 1311-1317.
Chicago
Davies, G., Rmali, K. A., Watkins, G., Mansel, R. E., Mason, M. D., Jiang, W. G."Elevated levels of tumour endothelial marker-8 in human breast cancer and its clinical significance". International Journal of Oncology 29, no. 5 (2006): 1311-1317. https://doi.org/10.3892/ijo.29.5.1311