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MONOCLONAL-ANTIBODY AGAINST DOXORUBICIN (DXR) - SOME CHARACTERISTICS AND UTILIZATION FOR DXR-IMMUNOASSAY

  • Authors:
    • K OHKAWA
    • T HATANO
    • K TAKADA
    • Y TSUKADA
    • M MATSUDA

  • View Affiliations

  • Published online on: July 1, 1993     https://doi.org/10.3892/ijo.3.1.43
  • Pages: 43-46
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Abstract

To establish a rapid, specific, sensitive and simple assay method for doxorubicin (DXR) in body fluid, monoclonal antibodies (MAbs) against DXR were generated by immunizing mice with keyhole limpet hemocyanin-DXR conjugate, cell fusion, and a one step, time saving screening ELISA method using aminoplate-coupled DXR via a glutaraldehyde bridge as solid phase antigen. Inhibition ELISA for DXR-immunoassay was established using anti-DXR MAb of the best producer (2E9) and aminoplate-coupled DXR as antigen and DXR ranging from 50 pg to 50 ng in the body fluid or in the cell extract could be detected. MAb 2E9 cross-reacted to various degrees to anthracycline compounds, such as some DXR analogues and derivatives, but did not recognize anthracene and anthraquinone structures.

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July 1993
Volume 3 Issue 1

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
OHKAWA K, HATANO T, TAKADA K, TSUKADA Y and MATSUDA M: MONOCLONAL-ANTIBODY AGAINST DOXORUBICIN (DXR) - SOME CHARACTERISTICS AND UTILIZATION FOR DXR-IMMUNOASSAY. Int J Oncol 3: 43-46, 1993
APA
OHKAWA, K., HATANO, T., TAKADA, K., TSUKADA, Y., & MATSUDA, M. (1993). MONOCLONAL-ANTIBODY AGAINST DOXORUBICIN (DXR) - SOME CHARACTERISTICS AND UTILIZATION FOR DXR-IMMUNOASSAY. International Journal of Oncology, 3, 43-46. https://doi.org/10.3892/ijo.3.1.43
MLA
OHKAWA, K., HATANO, T., TAKADA, K., TSUKADA, Y., MATSUDA, M."MONOCLONAL-ANTIBODY AGAINST DOXORUBICIN (DXR) - SOME CHARACTERISTICS AND UTILIZATION FOR DXR-IMMUNOASSAY". International Journal of Oncology 3.1 (1993): 43-46.
Chicago
OHKAWA, K., HATANO, T., TAKADA, K., TSUKADA, Y., MATSUDA, M."MONOCLONAL-ANTIBODY AGAINST DOXORUBICIN (DXR) - SOME CHARACTERISTICS AND UTILIZATION FOR DXR-IMMUNOASSAY". International Journal of Oncology 3, no. 1 (1993): 43-46. https://doi.org/10.3892/ijo.3.1.43