ASSESSMENT OF CELL-PROLIFERATION KINETICS IN FAMILIAL ADENOMATOUS POLYPOSIS BY PCNA IMMUNOSTAINING

  • Authors:
    • R YOSHIKAWA
    • T HATADA
    • M KUSUNOKI
    • T YAMAMURA
    • J UTSUNOMIYA
  • View Affiliations

  • Published online on: August 1, 1993     https://doi.org/10.3892/ijo.3.2.341
  • Pages: 341-345
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Abstract

Cell proliferation was assessed using immunostaining for proliferative cell nuclear antigen in 10 patients with familial adenomatous polyposis (10 normal-appearing mucosa, 19 adenoma and 3 adenocarcinoma specimens) and 6 control subjects (rectosigmoid mucosal biopsies). The total labeling index (percentage ratio of the number of labeled cells to the total number of cells in a column) was 31.70+/-9.32% (mean+/-SD) in adenocarcinoma, 27.95+/-4.24% in adenoma (p<0.001 versus normal-appearing mucosa), 19.54+/-3.46% in normal-appearing mucosa (p<0.01 versus control mucosa) and 13.73+/-5.19% in control mucosa. Adenomas showed hyperproliferation and upward expansion of the proliferative zone. When adenomas were classified by size, their diameter was related to the proliferative activity (r=0.73, p<0.01). The normal-appearing mucosa already showed changes in cell proliferation. In an animal study, a linear correlation between the labeling index for proliferative nuclear cell antigen and that for bromodeoxyuridine was demonstrated in rat colonic mucosa (r=0.96, p<0.001). These results suggest that immunostaining for proliferative cell nuclear antigen can be used as a convenient and precise method of studying cell proliferation kinetics and that the increase of the proliferative activity of adenomas in proportion to their size provides some support for the adenoma-carcinoma sequence.

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August 1993
Volume 3 Issue 2

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
YOSHIKAWA R, HATADA T, KUSUNOKI M, YAMAMURA T and UTSUNOMIYA J: ASSESSMENT OF CELL-PROLIFERATION KINETICS IN FAMILIAL ADENOMATOUS POLYPOSIS BY PCNA IMMUNOSTAINING. Int J Oncol 3: 341-345, 1993
APA
YOSHIKAWA, R., HATADA, T., KUSUNOKI, M., YAMAMURA, T., & UTSUNOMIYA, J. (1993). ASSESSMENT OF CELL-PROLIFERATION KINETICS IN FAMILIAL ADENOMATOUS POLYPOSIS BY PCNA IMMUNOSTAINING. International Journal of Oncology, 3, 341-345. https://doi.org/10.3892/ijo.3.2.341
MLA
YOSHIKAWA, R., HATADA, T., KUSUNOKI, M., YAMAMURA, T., UTSUNOMIYA, J."ASSESSMENT OF CELL-PROLIFERATION KINETICS IN FAMILIAL ADENOMATOUS POLYPOSIS BY PCNA IMMUNOSTAINING". International Journal of Oncology 3.2 (1993): 341-345.
Chicago
YOSHIKAWA, R., HATADA, T., KUSUNOKI, M., YAMAMURA, T., UTSUNOMIYA, J."ASSESSMENT OF CELL-PROLIFERATION KINETICS IN FAMILIAL ADENOMATOUS POLYPOSIS BY PCNA IMMUNOSTAINING". International Journal of Oncology 3, no. 2 (1993): 341-345. https://doi.org/10.3892/ijo.3.2.341