IL-3 can not replace GM-CSF in inducing human monocytes to differentiate into Langerhans cells

  • Authors:
    • Tetsunori Shibasaki
    • Naoyuki Katayama
    • Kohshi Ohishi
    • Atsushi Fujieda
    • Fumihiko Monma
    • Kazuhiro Nishi
    • Masahiro Masuya
    • Hiroshi Shiku
  • View Affiliations

  • Published online on: March 1, 2007     https://doi.org/10.3892/ijo.30.3.549
  • Pages: 549-555
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Abstract

Receptors for granulocyte-macrophage colony-stimulating factor (GM-CSF) and interleukin-3 (IL-3) share a common β subunit. We recently reported that GM-CSF acts in concert with transforming growth factor-β1 (TGF-β1) and Notch ligand Delta-1 (Delta-1) to promote the differentiation of human blood monocytes into Langerhans cells. In the present study, we examined whether IL-3, in place of GM-CSF, can induce the development of Langerhans cells from blood monocytes in the presence of TGF-β1 and Delta-1, because the IL-3 receptor α chain was substantially expressed on monocytes. However, the generation of Langerhans cells was not obtained by the combination of IL-3, TGF-β1 and Delta-1, even though GM-CSF and IL-3 exhibited a similar effect with respect to the differentiation of monocytes into macrophages and dendritic cells. The addition of GM-CSF to the culture supplemented with IL-3, TGF-β1 and Delta-1 restored the differentiation of monocytes toward Langerhans cells. A microarray analysis revealed that a number of genes including Langerhans cell markers, E-cadherin and Langerin, were specifically expressed in cells from GM-CSF-containing cultures but not in those from IL-3-containing cultures. These data suggest that IL-3 can not replace GM-CSF to induce the differentiation of human monocytes into Langerhans cells in culture.

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March 2007
Volume 30 Issue 3

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Shibasaki T, Katayama N, Ohishi K, Fujieda A, Monma F, Nishi K, Masuya M and Shiku H: IL-3 can not replace GM-CSF in inducing human monocytes to differentiate into Langerhans cells. Int J Oncol 30: 549-555, 2007.
APA
Shibasaki, T., Katayama, N., Ohishi, K., Fujieda, A., Monma, F., Nishi, K. ... Shiku, H. (2007). IL-3 can not replace GM-CSF in inducing human monocytes to differentiate into Langerhans cells. International Journal of Oncology, 30, 549-555. https://doi.org/10.3892/ijo.30.3.549
MLA
Shibasaki, T., Katayama, N., Ohishi, K., Fujieda, A., Monma, F., Nishi, K., Masuya, M., Shiku, H."IL-3 can not replace GM-CSF in inducing human monocytes to differentiate into Langerhans cells". International Journal of Oncology 30.3 (2007): 549-555.
Chicago
Shibasaki, T., Katayama, N., Ohishi, K., Fujieda, A., Monma, F., Nishi, K., Masuya, M., Shiku, H."IL-3 can not replace GM-CSF in inducing human monocytes to differentiate into Langerhans cells". International Journal of Oncology 30, no. 3 (2007): 549-555. https://doi.org/10.3892/ijo.30.3.549