Increased class 3 semaphorin expression modulates the invasive and adhesive properties of prostate cancer cells

  • Authors:
    • Jeffery G. Herman
    • Gary G. Meadows
  • View Affiliations

  • Published online on: May 1, 2007     https://doi.org/10.3892/ijo.30.5.1231
  • Pages: 1231-1238
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Abstract

The class 3 semaphorins, sema3A and sema3C, provide important guidance cues in cell development and in cancer; however, the role of these semaphorins in prostate cancer is not known. We report here that sema3A transfected cells exhibit decreased invasion and adhesion in Matrigel-based assays and that sema3C transfected cells exhibit increased invasive and adhesive characteristics. Important adhesion proteins were differentially modulated in sema3A and sema3C cells in a manner consistent with their subsequent invasive and adhesive characteristics. E-cadherin expression as determined by Western blot analysis was strongly upregulated in sema3A transfected cells, but strongly downregulated in sema3C transfected cells compared to untransfected and mock empty vector-transfected PC-3 cells. β-catenin levels were not changed in sema3A transfected cells; however, sema3C transfected cells had lower expression of this protein. Sema3C transfected cells exhibited greater cellular membrane expression of certain α integrins as compared to untransfected and sema3A transfected cells, a characteristic associated with increased adhesion and invasion. These data indicate that the invasive ability of sema3A and sema3C transfected PC-3 cells is, in part, correlated with adhesion protein expression and adhesive ability to constituents of neighboring cells and the extracellular matrix.

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May 2007
Volume 30 Issue 5

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Herman JG and Herman JG: Increased class 3 semaphorin expression modulates the invasive and adhesive properties of prostate cancer cells. Int J Oncol 30: 1231-1238, 2007
APA
Herman, J.G., & Herman, J.G. (2007). Increased class 3 semaphorin expression modulates the invasive and adhesive properties of prostate cancer cells. International Journal of Oncology, 30, 1231-1238. https://doi.org/10.3892/ijo.30.5.1231
MLA
Herman, J. G., Meadows, G. G."Increased class 3 semaphorin expression modulates the invasive and adhesive properties of prostate cancer cells". International Journal of Oncology 30.5 (2007): 1231-1238.
Chicago
Herman, J. G., Meadows, G. G."Increased class 3 semaphorin expression modulates the invasive and adhesive properties of prostate cancer cells". International Journal of Oncology 30, no. 5 (2007): 1231-1238. https://doi.org/10.3892/ijo.30.5.1231