The benzene metabolite hydroquinone enhances G2-chromosomal radiosensitivity by inducing a less-efficient G2-M-checkpoint in irradiated lymphocytes

  • Authors:
    • Vasiliki I. Hatzi
    • Georgia I. Terzoudi
    • Gabriel E. Pantelias
    • Chara Spiliopoulou
    • Vasilios Makropoulos
  • View Affiliations

  • Published online on: July 1, 2007     https://doi.org/10.3892/ijo.31.1.145
  • Pages: 145-152
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

The hypothesis tested is that a 24-h pre-irradiation-exposure of peripheral blood lymphocytes (PBL) to the benzene metabolite hydroquinone (HQ), at doses that are non-acutely toxic (5 µM), induces a less efficient G2-M-checkpoint and enhances the G2-chromosomal radiosensitivity in a statistically significant manner (p<0.01). A less efficient G2-M-checkpoint may allow the transition of damaged cells from G2- to M-phase and experimental data in the present work support this hypothesis. In fact HQ sensitizes lymphocytes obtained from healthy donors, as they exhibit increased G2-chromosomal radiosensitivity which interestingly is similar to that observed in cases of radiosensitive cancer-prone individuals. This finding is important since a deficiency in cell cycle checkpoints and an increase in G2-chromosomal radiosensitivity are linked to chromosomal instability, cancer proneness and the development of leukemia. The observed chromosome radiosensitization may be a consequence either of an effect of HQ on the initial induction of radiation-induced chromosomal aberrations, or on the DNA repair capacity of the cells, or it may be linked to HQ-induced alterations in the cell cycle and feedback control mechanism during the G2- to M-phase transition. In order to elucidate which is the mechanism involved, conventional cytogenetics and premature chromosome condensation (PCC) methodologies were applied. The experimental data obtained support the hypothesis that HQ increases G2-chromosomal radiosensitivity in human peripheral blood lymphocytes by inducing a less efficient G2-M-checkpoint, facilitating thus the transition of damaged cells from G2- to M-phase.

Related Articles

Journal Cover

July 2007
Volume 31 Issue 1

Print ISSN: 1019-6439
Online ISSN:1791-2423

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Hatzi VI, Terzoudi GI, Pantelias GE, Spiliopoulou C and Makropoulos V: The benzene metabolite hydroquinone enhances G2-chromosomal radiosensitivity by inducing a less-efficient G2-M-checkpoint in irradiated lymphocytes. Int J Oncol 31: 145-152, 2007
APA
Hatzi, V.I., Terzoudi, G.I., Pantelias, G.E., Spiliopoulou, C., & Makropoulos, V. (2007). The benzene metabolite hydroquinone enhances G2-chromosomal radiosensitivity by inducing a less-efficient G2-M-checkpoint in irradiated lymphocytes. International Journal of Oncology, 31, 145-152. https://doi.org/10.3892/ijo.31.1.145
MLA
Hatzi, V. I., Terzoudi, G. I., Pantelias, G. E., Spiliopoulou, C., Makropoulos, V."The benzene metabolite hydroquinone enhances G2-chromosomal radiosensitivity by inducing a less-efficient G2-M-checkpoint in irradiated lymphocytes". International Journal of Oncology 31.1 (2007): 145-152.
Chicago
Hatzi, V. I., Terzoudi, G. I., Pantelias, G. E., Spiliopoulou, C., Makropoulos, V."The benzene metabolite hydroquinone enhances G2-chromosomal radiosensitivity by inducing a less-efficient G2-M-checkpoint in irradiated lymphocytes". International Journal of Oncology 31, no. 1 (2007): 145-152. https://doi.org/10.3892/ijo.31.1.145