Immunohistochemical detection of the lymphatic marker podoplanin in diverse types of human cancer cells using a novel antibody

  • Authors:
    • Tomoya Kono
    • Masayuki Shimoda
    • Meiko Takahashi
    • Kyoichi Matsumoto
    • Takanobu Yoshimoto
    • Mika Mizutani
    • Chiharu Tabata
    • Kae Okoshi
    • Hiromi Wada
    • Hajime Kubo
  • View Affiliations

  • Published online on: September 1, 2007     https://doi.org/10.3892/ijo.31.3.501
  • Pages: 501-508
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Abstract

The mucin-type transmembrane glycoprotein podoplanin (also known as T1α, gp38 or Aggrus) is well established as one of the lymphatic-specific markers. Podoplanin was also reported to be associated with tumor-induced platelet aggregation and tumor metastasis. Here, we generated a novel monoclonal antibody (clone; 7B10) that specifically recognized human podoplanin, as assessed by enzyme-linked immunosorbent assay, Western blot analyses, immunohistochemistry and flow cytometry. 7B10 stained not only lymphatic vessels but also type I alveolar cells in the lung, podocytes in the kidney and myoepithelial cells in the breast. In addition, podoplanin expression was analyzed by immunostaining on tissue microarrays that included 12 different cancer types using 7B10. Consequently, we found that podoplanin was expressed by cancer cells derived from esophagus, lung, liver, colon and breast, as well as lymphatic endothelial cells. These findings suggest a potential role of podoplanin in tumor progression in diverse types of human cancers.

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September 2007
Volume 31 Issue 3

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Kono T, Shimoda M, Takahashi M, Matsumoto K, Yoshimoto T, Mizutani M, Tabata C, Okoshi K, Wada H, Kubo H, Kubo H, et al: Immunohistochemical detection of the lymphatic marker podoplanin in diverse types of human cancer cells using a novel antibody. Int J Oncol 31: 501-508, 2007.
APA
Kono, T., Shimoda, M., Takahashi, M., Matsumoto, K., Yoshimoto, T., Mizutani, M. ... Kubo, H. (2007). Immunohistochemical detection of the lymphatic marker podoplanin in diverse types of human cancer cells using a novel antibody. International Journal of Oncology, 31, 501-508. https://doi.org/10.3892/ijo.31.3.501
MLA
Kono, T., Shimoda, M., Takahashi, M., Matsumoto, K., Yoshimoto, T., Mizutani, M., Tabata, C., Okoshi, K., Wada, H., Kubo, H."Immunohistochemical detection of the lymphatic marker podoplanin in diverse types of human cancer cells using a novel antibody". International Journal of Oncology 31.3 (2007): 501-508.
Chicago
Kono, T., Shimoda, M., Takahashi, M., Matsumoto, K., Yoshimoto, T., Mizutani, M., Tabata, C., Okoshi, K., Wada, H., Kubo, H."Immunohistochemical detection of the lymphatic marker podoplanin in diverse types of human cancer cells using a novel antibody". International Journal of Oncology 31, no. 3 (2007): 501-508. https://doi.org/10.3892/ijo.31.3.501