Saw Palmetto induces growth arrest and apoptosis of androgen-dependent prostate cancer LNCaP cells via inactivation of STAT 3 and androgen receptor signaling

  • Authors:
    • Yang Yang
    • Takayuki Ikezoe
    • Zhixing Zheng
    • Hirokuni Taguchi
    • H. Phillip Koeffler
    • Wei-Guo Zhu
  • View Affiliations

  • Published online on: September 1, 2007     https://doi.org/10.3892/ijo.31.3.593
  • Pages: 593-600
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Abstract

PC-SPES is an eight-herb mixture that has an activity against prostate cancer. Recently, we purified Saw Palmetto (Serenoa repens) from PC-SPES and found that Saw Palmetto induced growth arrest of prostate cancer LNCaP, DU145, and PC3 cells with ED50s of approximately 2.0, 2.6, and 3.3 µl/ml, respectively, as measured by mitochondrial-dependent conversion of the the 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay. Saw Palmetto induced apoptosis of LNCaP cells in a time- and dose-dependent manner as measured by TUNEL assays. Also, Saw Palmetto increased the expression of p21waf1 and p53 protein in LNCaP cells. In addition, we found that Saw Palmetto down-regulated DHT- or IL-6-induced expression of prostate specific antigen in conjunction with down-regulation of the level of androgen receptor in the nucleus as measured by Western blot analysis. Moreover, Saw Palmetto down-regulated the IL-6-induced level of the phosphorylated form of STAT 3 in LNCaP cells. Furthermore, Saw Palmetto inhibited the growth of LNCaP cells present as tumor xenografts in BALB/c nude mice without adverse effect. These results indicate that Saw Palmetto might be useful for the treatment of individuals with prostate cancer.

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September 2007
Volume 31 Issue 3

Print ISSN: 1019-6439
Online ISSN:1791-2423

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APA
Yang, Y., Ikezoe, T., Zheng, Z., Taguchi, H., Koeffler, H.P., & Zhu, W. (2007). Saw Palmetto induces growth arrest and apoptosis of androgen-dependent prostate cancer LNCaP cells via inactivation of STAT 3 and androgen receptor signaling. International Journal of Oncology, 31, 593-600. https://doi.org/10.3892/ijo.31.3.593
MLA
Yang, Y., Ikezoe, T., Zheng, Z., Taguchi, H., Koeffler, H. P., Zhu, W."Saw Palmetto induces growth arrest and apoptosis of androgen-dependent prostate cancer LNCaP cells via inactivation of STAT 3 and androgen receptor signaling". International Journal of Oncology 31.3 (2007): 593-600.
Chicago
Yang, Y., Ikezoe, T., Zheng, Z., Taguchi, H., Koeffler, H. P., Zhu, W."Saw Palmetto induces growth arrest and apoptosis of androgen-dependent prostate cancer LNCaP cells via inactivation of STAT 3 and androgen receptor signaling". International Journal of Oncology 31, no. 3 (2007): 593-600. https://doi.org/10.3892/ijo.31.3.593