12-O-tetradecanoyl phorbol 13-acetate induces the expression of B7-DC, -H1, -H2, and -H3 in K562 cells

  • Authors:
    • Byeong-Churl Jang
    • Yu-Kyoung Park
    • In-Hak Choi
    • Sang-Pyo Kim
    • Jin-Bok Hwang
    • Won-Ki Baek
    • Min-Ho Suh
    • Kyu-Chul Mun
    • Seong-Il Suh
  • View Affiliations

  • Published online on: December 1, 2007     https://doi.org/10.3892/ijo.31.6.1439
  • Pages: 1439-1447
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Abstract

Induction of the B7 family molecules by 12-O-tetradecanoyl phorbol 13-acetate (TPA) has been reported, however, the mechanism by which TPA up-regulates these molecules remains poorly understood. In this study, the expression of B7-DC, -H1, -H2, and -H3 in response to TPA was markedly induced in K562 cells. TPA also induced activation of ERK, p38 mitogen-activated protein kinase (MAPK), JNK, phosphatidylinositol-3-kinase (PI-3K), or nuclear factor (NF)-κB. Pre-treatments with protein kinase C (PKC) inhibitors significantly inhibited TPA-induced expression of B7-DC, -H1, -H2, and -H3 mRNA as well as TPA-induced phosphorylation of ERK, p38 MAPK, JNK, and PI-3K. TPA-induced expression of B7-DC, -H1, -H2, and -H3 mRNA was abrogated by pre-treatments with inhibitors of ERK and p38 MAPK. However, inhibition of PI-3K and JNK only caused decrease of TPA-induced B7-DC mRNA and B7-H3 mRNA, respectively. TPA-induced degradation of IκB-α was markedly abrogated by treatments with PKC inhibitors, but not by treatments with inhibitors of ERK, p38 MAPK, JNK, or PI-3K. NF-κB inhibitors significantly attenuated the expression of B7-DC, -H1, -H2, and -H3 mRNA in response to TPA. These results suggest that TPA induces the expression of B7-DC, -H1, -H2, and -H3 mRNA in K562 cells via activation of PKC, ERK, p38 MAPK, and NF-κB. Distinctly, the expression of B7-DC mRNA and -H3 mRNA in response to TPA is also PI-3K- and JNK-dependent, respectively.

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December 2007
Volume 31 Issue 6

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Jang B, Park Y, Choi I, Kim S, Hwang J, Baek W, Suh M, Mun K and Suh S: 12-O-tetradecanoyl phorbol 13-acetate induces the expression of B7-DC, -H1, -H2, and -H3 in K562 cells. Int J Oncol 31: 1439-1447, 2007
APA
Jang, B., Park, Y., Choi, I., Kim, S., Hwang, J., Baek, W. ... Suh, S. (2007). 12-O-tetradecanoyl phorbol 13-acetate induces the expression of B7-DC, -H1, -H2, and -H3 in K562 cells. International Journal of Oncology, 31, 1439-1447. https://doi.org/10.3892/ijo.31.6.1439
MLA
Jang, B., Park, Y., Choi, I., Kim, S., Hwang, J., Baek, W., Suh, M., Mun, K., Suh, S."12-O-tetradecanoyl phorbol 13-acetate induces the expression of B7-DC, -H1, -H2, and -H3 in K562 cells". International Journal of Oncology 31.6 (2007): 1439-1447.
Chicago
Jang, B., Park, Y., Choi, I., Kim, S., Hwang, J., Baek, W., Suh, M., Mun, K., Suh, S."12-O-tetradecanoyl phorbol 13-acetate induces the expression of B7-DC, -H1, -H2, and -H3 in K562 cells". International Journal of Oncology 31, no. 6 (2007): 1439-1447. https://doi.org/10.3892/ijo.31.6.1439