The propeptide of cathepsin D increases proliferation, invasion and metastasis of breast cancer cells

  • Authors:
    • Sujata Saraswat Ohri
    • Aruna Vashishta
    • Mary Proctor
    • Martin Fusek
    • Vaclav Vetvicka
  • View Affiliations

  • Published online on: February 1, 2008     https://doi.org/10.3892/ijo.32.2.491
  • Pages: 491-498
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Expression and secretion of procathepsin D (pCD) increases proliferation, metastasis and progression of breast cancer but the structural moiety by which pCD exerts these effects is still ambiguous. Here, we present data on a series of pCD stable mutants to identify the pCD region that mediates this mitogenic effect. Mutations affecting the region of the activation peptide (AP) were studied together with catalytic and glycosylation mutants. Mitogenic effect was evaluated using in vitro invasion and proliferation assays and in vivo by determining the tumorigenic potential. The catalytic mutants and glycosylation mutants of pCD continued to display enhanced cell proliferation, invasion and tumorigenicity similar to stable transfectants of native pCD, suggesting that neither the proteolytic activity nor the sugar moieties contribute to the mitogenic effect. However, stable transfectants of pCD lacking its AP and with various mutations in the 27-44 amino acid region of AP, failed to show enhanced cell proliferation or invasion in vitro and tumor growth in vivo, establishing the importance of AP region. Our study concludes that the entire 27-44 amino acid region of AP is necessary for the stimulatory actions of pCD on breast cancer cells.

Related Articles

Journal Cover

February 2008
Volume 32 Issue 2

Print ISSN: 1019-6439
Online ISSN:1791-2423

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Ohri SS, Vashishta A, Proctor M, Fusek M and Vetvicka V: The propeptide of cathepsin D increases proliferation, invasion and metastasis of breast cancer cells. Int J Oncol 32: 491-498, 2008
APA
Ohri, S.S., Vashishta, A., Proctor, M., Fusek, M., & Vetvicka, V. (2008). The propeptide of cathepsin D increases proliferation, invasion and metastasis of breast cancer cells. International Journal of Oncology, 32, 491-498. https://doi.org/10.3892/ijo.32.2.491
MLA
Ohri, S. S., Vashishta, A., Proctor, M., Fusek, M., Vetvicka, V."The propeptide of cathepsin D increases proliferation, invasion and metastasis of breast cancer cells". International Journal of Oncology 32.2 (2008): 491-498.
Chicago
Ohri, S. S., Vashishta, A., Proctor, M., Fusek, M., Vetvicka, V."The propeptide of cathepsin D increases proliferation, invasion and metastasis of breast cancer cells". International Journal of Oncology 32, no. 2 (2008): 491-498. https://doi.org/10.3892/ijo.32.2.491