Finasteride induces apoptosis via Bcl-2, Bcl-xL, Bax and caspase-3 proteins in LNCaP human prostate cancer cell line

  • Authors:
    • Jesús M. Golbano
    • Pilar Lóppez-Aparicio
    • María N. Recio
    • Miguel A. Pérez-Albarsanz
  • View Affiliations

  • Published online on: April 1, 2008     https://doi.org/10.3892/ijo.32.4.919
  • Pages: 919-924
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Abstract

We investigated the effects of finasteride, a 5α-reductase inhibitor, on cell death machinery through the induction of apoptosis in an in vitro model for prostate cancer. Finasteride treatment of the LNCaP hormone-dependent human prostate cancer cell line caused the loss of cell viability and accelerated apoptosis in a concentration-dependent manner. The contents of immunoreactive procaspase-3 were examined by immunoblot analysis and the results suggest that the apoptosis induced by finasteride involves the increase of caspase-3 activity. Early cell changes that occur during apoptosis are associated with mitochondrial changes mediated by members of the Bcl-2 family of proteins. Therefore, Bcl-2, Bcl-xL and Bax were evaluated by the Western blot analysis. The immunoreactivity for pro-apoptotic Bax was markedly increased whereas antiapoptotic Bcl-2 and Bcl-xL expression was significantly reduced after incubation of cells with finasteride. These findings suggest that finasteride induces apoptosis in LNCaP cells via proteins of the Bcl-2 and caspase family.

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April 2008
Volume 32 Issue 4

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Golbano JM, Lóppez-Aparicio P, Recio MN and Pérez-Albarsanz MA: Finasteride induces apoptosis via Bcl-2, Bcl-xL, Bax and caspase-3 proteins in LNCaP human prostate cancer cell line. Int J Oncol 32: 919-924, 2008.
APA
Golbano, J.M., Lóppez-Aparicio, P., Recio, M.N., & Pérez-Albarsanz, M.A. (2008). Finasteride induces apoptosis via Bcl-2, Bcl-xL, Bax and caspase-3 proteins in LNCaP human prostate cancer cell line. International Journal of Oncology, 32, 919-924. https://doi.org/10.3892/ijo.32.4.919
MLA
Golbano, J. M., Lóppez-Aparicio, P., Recio, M. N., Pérez-Albarsanz, M. A."Finasteride induces apoptosis via Bcl-2, Bcl-xL, Bax and caspase-3 proteins in LNCaP human prostate cancer cell line". International Journal of Oncology 32.4 (2008): 919-924.
Chicago
Golbano, J. M., Lóppez-Aparicio, P., Recio, M. N., Pérez-Albarsanz, M. A."Finasteride induces apoptosis via Bcl-2, Bcl-xL, Bax and caspase-3 proteins in LNCaP human prostate cancer cell line". International Journal of Oncology 32, no. 4 (2008): 919-924. https://doi.org/10.3892/ijo.32.4.919