Differential expression of GHRH receptor and its splice variant 1 in human normal and malignant mucosa of the oesophagus and colon

  • Authors:
    • Florian Hohla
    • Angelika Moder
    • Ursula Mayrhauser
    • Cornelia Hauser-Kronberger
    • Andrew V. Schally
    • Jozsef L. Varga
    • Marta Zarandi
    • Stefan Buchholz
    • Rudolf Huber
    • Elmar Aigner
    • Markus Ritter
    • Christian Datz
  • View Affiliations

  • Published online on: July 1, 2008     https://doi.org/10.3892/ijo.33.1.137
  • Pages: 137-143
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Abstract

Recent evidence indicates that growth hormone-releasing hormone (GHRH) functions as a growth factor for gastrointestinal (GI) tumours. The tumourigenic effects of GHRH appear to be mediated by the splice variant 1 (SV-1) of GHRH receptor as well as the full length pituitary type receptor for GHRH (GHRH-R). We examined the protein and mRNA expression of GHRH-R and SV-1 in normal human tissues and tumours of the gastrointestinal (GI-) tract by immunohistochemical staining and reverse transcriptase (RT)-PCR. Squamous cells and squamous cell carcinoma of the oesophagus were negative for GHRH-R and SV-1, while Barrett's mucosa and adenocarcinomas of the oesophagus showed a strong expression of both receptors. The expression of GHRH-R was absent in normal colonic mucosa other than neuroendocrine cells (NE) and lining epithelium (LE) but strong in tubular adenomas of the colon, while the staining for SV-1 was absent in cells other than NE. However, the expression of both receptors was significantly increased in tubulovillous adenomas and colorectal cancers. No differences were seen in protein levels for both receptors between normal and neoplastic tissues of the stomach, pancreas and liver. Because of low mRNA levels for both receptors in all samples tested, only a qualitative assessment could be made. However, mRNA for GHRH-R and SV-1 showed a near-perfect correlation with the assessment of receptor proteins by immunostaining. Our study shows that in contrast to normal mucosa, transformed mucosa of the oesophagus and the colon expresses GHRH-R and SV-1. This aberrant expression of GHRH-R and SV-1 in oesophageal and colorectal malignancies may provide a molecular target for a therapeutic approach based on GHRH antagonists.

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July 2008
Volume 33 Issue 1

Print ISSN: 1019-6439
Online ISSN:1791-2423

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APA
Hohla, F., Moder, A., Mayrhauser, U., Hauser-Kronberger, C., Schally, A.V., Varga, J.L. ... Datz, C. (2008). Differential expression of GHRH receptor and its splice variant 1 in human normal and malignant mucosa of the oesophagus and colon. International Journal of Oncology, 33, 137-143. https://doi.org/10.3892/ijo.33.1.137
MLA
Hohla, F., Moder, A., Mayrhauser, U., Hauser-Kronberger, C., Schally, A. V., Varga, J. L., Zarandi, M., Buchholz, S., Huber, R., Aigner, E., Ritter, M., Datz, C."Differential expression of GHRH receptor and its splice variant 1 in human normal and malignant mucosa of the oesophagus and colon". International Journal of Oncology 33.1 (2008): 137-143.
Chicago
Hohla, F., Moder, A., Mayrhauser, U., Hauser-Kronberger, C., Schally, A. V., Varga, J. L., Zarandi, M., Buchholz, S., Huber, R., Aigner, E., Ritter, M., Datz, C."Differential expression of GHRH receptor and its splice variant 1 in human normal and malignant mucosa of the oesophagus and colon". International Journal of Oncology 33, no. 1 (2008): 137-143. https://doi.org/10.3892/ijo.33.1.137