Caspase inhibitor decreases apoptosis in pyrogallol-treated lung cancer Calu-6 cells via the prevention of GSH depletion

  • Authors:
    • Yong Hwan Han
    • Suhn Hee Kim
    • Sung Zoo Kim
    • Woo Hyun Park
  • View Affiliations

  • Published online on: November 1, 2008     https://doi.org/10.3892/ijo_00000099
  • Pages: 1099-1105
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Abstract

Pyrogallol (PG) is a polyphenol compound and is known to be an O2·- generator. In the present study, we evaluated the anti-apoptotic effects of caspase inhibitors in relation to changes in reactive oxygen species (ROS) and glutathione (GSH) levels in PG-treated human pulmonary adenocarcinoma Calu-6 cells. Treatment with 50 µM PG inhibited the growth of Calu-6 cells ≈60% and induced apoptosis ≈17% at 24 h, accompanied by mitochondrial membrane potential loss (ΔΨm). Treatment with pan-caspase inhibitor (Z-VAD-FMK), caspase-3 inhibitor (Z-DEVD-FMK), caspase-8 inhibitor (Z-IETD-FMK) and caspase-9 inhibitor (Z-LEHD-FMK) significantly prevented apoptosis in PG-treated Calu-6 cells at 24 h. PG increased the ROS and depleted GSH contents in Calu-6 cells. Treatment with each caspase inhibitor did not significantly change the ROS and GSH levels in PG-treated Calu-6 cells at 24 h. However, Z-VAD significantly prevented GSH depletion in PG-treated Calu-6 cells at the late time phase of 72 h. Conclusively, the anti-apoptotic effect of caspase inhibitor on PG-induced Calu-6 cell death was closely related to changes in GSH content rather than ROS levels.

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November 2008
Volume 33 Issue 5

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Han YH, Kim SH, Kim SZ and Park WH: Caspase inhibitor decreases apoptosis in pyrogallol-treated lung cancer Calu-6 cells via the prevention of GSH depletion. Int J Oncol 33: 1099-1105, 2008.
APA
Han, Y.H., Kim, S.H., Kim, S.Z., & Park, W.H. (2008). Caspase inhibitor decreases apoptosis in pyrogallol-treated lung cancer Calu-6 cells via the prevention of GSH depletion. International Journal of Oncology, 33, 1099-1105. https://doi.org/10.3892/ijo_00000099
MLA
Han, Y. H., Kim, S. H., Kim, S. Z., Park, W. H."Caspase inhibitor decreases apoptosis in pyrogallol-treated lung cancer Calu-6 cells via the prevention of GSH depletion". International Journal of Oncology 33.5 (2008): 1099-1105.
Chicago
Han, Y. H., Kim, S. H., Kim, S. Z., Park, W. H."Caspase inhibitor decreases apoptosis in pyrogallol-treated lung cancer Calu-6 cells via the prevention of GSH depletion". International Journal of Oncology 33, no. 5 (2008): 1099-1105. https://doi.org/10.3892/ijo_00000099