Establishment of a novel neuroblastoma mouse model

  • Authors:
    • Hiroshi Iwakura
    • Hiroyuki Ariyasu
    • Naotetsu Kanamoto
    • Kiminori Hosoda
    • Kazuwa Nakao
    • Kenji Kangawa
    • Takashi Akamizu
  • View Affiliations

  • Published online on: December 1, 2008     https://doi.org/10.3892/ijo_00000109
  • Pages: 1195-1199
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Neuroblastoma is the most common childhood cancer, which arises from sympathetic neural precursors. Because the prognosis of advanced neuroblastoma is known to be poor, developments of new anti-cancer drugs are desperately needed. For screening of therapeutic drugs for neuroblastoma, genetically engineered animal models would be useful. In an attempt to obtain transgenic mice carrying simian virus 40 T-antigen gene under control of tetracycline responsive elements with cytomegalovirus promoter, we found one line of mice exhibiting bilateral adrenal tumors by leakage expression of T-antigen in adrenal gland. These adrenal tumors contained small round tumor cells with increased N/C ratio, showing chromogranin A and neuron specific enolase-like immunoreactivity. By electron microscopy, tumor cells containing neuritic processes with synaptic vesicles surrounding them were observed. The plasma levels of dopamine were significantly elevated in these transgenic mice. MYCN expression levels were significantly elevated in these tumors. These findings indicated that the adrenal tumor was a neuroblastoma. This mouse model would be a useful tool for development of chemotherapeutic drugs and understanding the etiology of neuroblastoma.

Related Articles

Journal Cover

December 2008
Volume 33 Issue 6

Print ISSN: 1019-6439
Online ISSN:1791-2423

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Iwakura H, Ariyasu H, Kanamoto N, Hosoda K, Nakao K, Kangawa K and Akamizu T: Establishment of a novel neuroblastoma mouse model. Int J Oncol 33: 1195-1199, 2008.
APA
Iwakura, H., Ariyasu, H., Kanamoto, N., Hosoda, K., Nakao, K., Kangawa, K., & Akamizu, T. (2008). Establishment of a novel neuroblastoma mouse model. International Journal of Oncology, 33, 1195-1199. https://doi.org/10.3892/ijo_00000109
MLA
Iwakura, H., Ariyasu, H., Kanamoto, N., Hosoda, K., Nakao, K., Kangawa, K., Akamizu, T."Establishment of a novel neuroblastoma mouse model". International Journal of Oncology 33.6 (2008): 1195-1199.
Chicago
Iwakura, H., Ariyasu, H., Kanamoto, N., Hosoda, K., Nakao, K., Kangawa, K., Akamizu, T."Establishment of a novel neuroblastoma mouse model". International Journal of Oncology 33, no. 6 (2008): 1195-1199. https://doi.org/10.3892/ijo_00000109