Over- and under-expressed microRNAs in human colorectal cancer

  • Authors:
    • Kazuo Motoyama
    • Hiroshi Inoue
    • Yasushi Takatsuno
    • Fumiaki Tanaka
    • Koshi Mimori
    • Hiroyuki Uetake
    • Kenichi Sugihara
    • Masaki Mori
  • View Affiliations

  • Published online on: Wednesday, April 1, 2009
  • Pages: 1069-1075 DOI: 10.3892/ijo_00000233

Abstract

MicroRNAs (miRNAs) constitute a class of small (21-23 nucleotides) noncoding RNAs that function as post-transcriptional gene regulators. It is becoming increasingly clear that altered miRNA expression correlates with the pathogenesis of cancers. The purpose of this study was to determine the up-regulated miRNAs in human colorectal cancer. Total RNA was isolated from cancer tissues and corresponding noncancerous tissues from surgically resected colorectal cancers. The expression profiles of miRNAs were determined using a miRNA microarray containing 455 human miRNA probes. The expression status of selected miRNAs in paired clinical samples was then investigated by real-time RT-PCR. Twenty-one miRNAs were identified by miRNA array analysis as overexpressed in colorectal cancer tissues compared to normal epithelial tissues. Among them, the expression of miR-31, miR-183, miR-17-5p, miR-18a, miR-20a and miR-92 were confirmed to be significantly higher in cancer tissues than in normal tissues (P<0.05). In contrast, the expression of miR-143 and miR-145 in cancer tissues were significantly lower than in normal tissues (P<0.05). The miR-18a overexpression group tended to have a poorer clinical prognosis than the low expression group (P=0.07). We identified miRNAs that were overexpressed or under-expressed in colorectal cancers and which may be correlated with colorectal carcinogenesis.

Related Articles

Journal Cover

April 2009
Volume 34 Issue 4

Print ISSN: 1019-6439
Online ISSN:1791-2423

2015 Impact Factor: 3.018
Ranked #30/211 Oncology
(total number of cites)

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
APA
Motoyama, K., Inoue, H., Takatsuno, Y., Tanaka, F., Mimori, K., Uetake, H. ... Mori, M. (2009). Over- and under-expressed microRNAs in human colorectal cancer. International Journal of Oncology, 34, 1069-1075. http://dx.doi.org/10.3892/ijo_00000233
MLA
Motoyama, K., Inoue, H., Takatsuno, Y., Tanaka, F., Mimori, K., Uetake, H., Sugihara, K., Mori, M."Over- and under-expressed microRNAs in human colorectal cancer". International Journal of Oncology 34.4 (2009): 1069-1075.
Chicago
Motoyama, K., Inoue, H., Takatsuno, Y., Tanaka, F., Mimori, K., Uetake, H., Sugihara, K., Mori, M."Over- and under-expressed microRNAs in human colorectal cancer". International Journal of Oncology 34, no. 4 (2009): 1069-1075. http://dx.doi.org/10.3892/ijo_00000233