Co-suppression of miR-221/222 cluster suppresses human glioma cell growth by targeting p27kip1 in vitro and in vivo

  • Authors:
    • Chunzhi Zhang
    • Chunsheng Kang
    • Yongping You
    • Peiyu Pu
    • Weidong Yang
    • Peng Zhao
    • Guangxiu Wang
    • Anling Zhang
    • Zhifan Jia
    • Lei Han
    • Hao Jiang
  • View Affiliations

  • Published online on: June 1, 2009     https://doi.org/10.3892/ijo_00000296
  • Pages: 1653-1660
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Abstract

MicroRNAs are short regulatory RNAs that negatively modulate protein expression at a post-transcriptional level. Emerging evidence suggests that altered regulation of miRNA may be involved in the pathogenesis of several types of cancers. In the current study, an inverse relationship between the expression of miR-221/miR-222 and the cell cycle inhibitor p27Kip1 was identified in U251 glioma cells. Co-suppression of miR-221/222 directly resulted in the up-regulation of p27Kip1 in the tested cells, consequently, affects their growth potential by reducing a G1 to S shift in the cell cycle. Consistently, miR-221/222 knocked-down through antisense 2'-OME-oligonucleotides increased p27Kip1 in U251 glioma subcutaneous mice and strongly reduced tumor growth in vivo through up regulation of p27Kip1. Our results suggest that miR-221/222 is a regulator of the tumor suppressor gene p27Kip1, and co-suppression of miR-221/222 expression in advanced gliomas may inhibit glioma cell proliferation by a mechanism involving the up-regulation of p27Kip1 in vitro and in vivo.

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June 2009
Volume 34 Issue 6

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Zhang C, Kang C, You Y, Pu P, Yang W, Zhao P, Wang G, Zhang A, Jia Z, Han L, Han L, et al: Co-suppression of miR-221/222 cluster suppresses human glioma cell growth by targeting p27kip1 in vitro and in vivo. Int J Oncol 34: 1653-1660, 2009
APA
Zhang, C., Kang, C., You, Y., Pu, P., Yang, W., Zhao, P. ... Jiang, H. (2009). Co-suppression of miR-221/222 cluster suppresses human glioma cell growth by targeting p27kip1 in vitro and in vivo. International Journal of Oncology, 34, 1653-1660. https://doi.org/10.3892/ijo_00000296
MLA
Zhang, C., Kang, C., You, Y., Pu, P., Yang, W., Zhao, P., Wang, G., Zhang, A., Jia, Z., Han, L., Jiang, H."Co-suppression of miR-221/222 cluster suppresses human glioma cell growth by targeting p27kip1 in vitro and in vivo". International Journal of Oncology 34.6 (2009): 1653-1660.
Chicago
Zhang, C., Kang, C., You, Y., Pu, P., Yang, W., Zhao, P., Wang, G., Zhang, A., Jia, Z., Han, L., Jiang, H."Co-suppression of miR-221/222 cluster suppresses human glioma cell growth by targeting p27kip1 in vitro and in vivo". International Journal of Oncology 34, no. 6 (2009): 1653-1660. https://doi.org/10.3892/ijo_00000296