Negative regulation of TIMP1 is mediated by transcription factor TWIST1

  • Authors:
    • Hirohiko Okamura
    • Kaya Yoshida
    • Tatsuji Haneji
  • View Affiliations

  • Published online on: Wednesday, July 1, 2009
  • Pages: 181-186 DOI: 10.3892/ijo_00000327

Abstract

TWIST1 is involved in tumor invasion and metastasis by promoting epithelial-to-mesenchymal transition. However, the molecular target of TWIST1 involving this mechanism is poorly understood. To identify the TWIST1-regulated genes, we established the Saos-2 cells stably expressing TWIST1 gene by transfecting TWIST1 cDNA into the cells and performed a differential display approach by using annealing control primers. Here, we report that one of the genes that were down-regulated in TWIST1 expressing cells is predicted to be TIMP1. TIMP1 has been reported as the naturally occurring specific inhibitor of matrix metalloproteinases (MMPs). Overexpression of TWIST1 gene suppressed the expression of TIMP1 mRNA but had no effect on TIMP2 and MMP-2 expression, as determined by semi-quantitative RT-PCR. We showed that TWIST1 was up-regulated in SCCBHY cells, which have a strong capacity of invasion into mandibular bone compared with SCCHN cells. Our present results suggest that TWIST1 is involved in tumor invasion by regulating the expression of TIMP1.

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July 2009
Volume 35 Issue 1

Print ISSN: 1019-6439
Online ISSN:1791-2423

2015 Impact Factor: 3.018
Ranked #30/211 Oncology
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APA
Okamura, H., Yoshida, K., & Haneji, T. (2009). Negative regulation of TIMP1 is mediated by transcription factor TWIST1. International Journal of Oncology, 35, 181-186. http://dx.doi.org/10.3892/ijo_00000327
MLA
Okamura, H., Yoshida, K., Haneji, T."Negative regulation of TIMP1 is mediated by transcription factor TWIST1". International Journal of Oncology 35.1 (2009): 181-186.
Chicago
Okamura, H., Yoshida, K., Haneji, T."Negative regulation of TIMP1 is mediated by transcription factor TWIST1". International Journal of Oncology 35, no. 1 (2009): 181-186. http://dx.doi.org/10.3892/ijo_00000327