MLH1 protects from resistance acquisition by the histone deacetylase inhibitor trichostatin A in colon tumor cells

  • Authors:
    • Patrick Imesch
    • Konstantin J. Dedes
    • Michele Furlato
    • Daniel Fink
    • Andre Fedier
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  • Published online on: September 1, 2009     https://doi.org/10.3892/ijo_00000375
  • Pages: 631-640
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Abstract

The antineoplastic activity of HDAC inhibitors is an unquestionable property of these compounds, but recent studies in tumor cells have revealed the potential of HDAC inhibitors (e.g., suberoylanilide hydroxamic acid SAHA, valproic acid VPA) to cause acquisition of HDAC inhibitor resistance. We report that trichostatin A (TSA), an HDAC inhibitor structurally related to SAHA, causes the acquisition of multidrug resistance transporter-independent and irreversible 3-fold resistance to TSA in MLH1-deficient (absent MLH1 protein expression) but not in MLH1-proficient (expressing MLH1 protein) HCT116 colon tumor cells. This MLH1-deficient subline selected for TSA resistance by stepwise exposures to increasing TSA concentrations exhibited failure in the accumulation of acetylated histones, in p21 induction, and in apoptosis activation. These are cellular responses normally seen in tumor cells treated with HDAC inhibitors. Whereas the absence of acetyl-histone accumulation did not correlate with altered HDAC activity, the absence of apoptosis correlated with reduced expression of (pro-apoptotic) Bax. This TSA-resistant subline was cross-resistant to SAHA and VPA but not to ‘classic’ non-HDAC inhibitor-type anticancer agents such as docetaxel and doxorubicin. These herein presented results expand on a previous study reporting HDAC inhibitor resistance acquisition by SAHA which was independent of the MLH1 expression status. Taken together, the present study identifies TSA, besides SAHA and VPA, as another potential causative of HDAC inhibitor resistance acquisition specifically in MLH1-deficient HCT116 colon tumor cells, and it reveals a possible function of MLH1 protein in protecting colon tumor cells from resistance acquisition by TSA.

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September 2009
Volume 35 Issue 3

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Imesch P, Dedes KJ, Furlato M, Fink D and Fedier A: MLH1 protects from resistance acquisition by the histone deacetylase inhibitor trichostatin A in colon tumor cells. Int J Oncol 35: 631-640, 2009
APA
Imesch, P., Dedes, K.J., Furlato, M., Fink, D., & Fedier, A. (2009). MLH1 protects from resistance acquisition by the histone deacetylase inhibitor trichostatin A in colon tumor cells. International Journal of Oncology, 35, 631-640. https://doi.org/10.3892/ijo_00000375
MLA
Imesch, P., Dedes, K. J., Furlato, M., Fink, D., Fedier, A."MLH1 protects from resistance acquisition by the histone deacetylase inhibitor trichostatin A in colon tumor cells". International Journal of Oncology 35.3 (2009): 631-640.
Chicago
Imesch, P., Dedes, K. J., Furlato, M., Fink, D., Fedier, A."MLH1 protects from resistance acquisition by the histone deacetylase inhibitor trichostatin A in colon tumor cells". International Journal of Oncology 35, no. 3 (2009): 631-640. https://doi.org/10.3892/ijo_00000375