Immunotherapy of breast cancer by single delivery with rAAV2-mediated interleukin-15 expression

  • Authors:
    • Yung-Luen Yu
    • Chyou-Wei Wei
    • Yi-Lin Chen
    • Mark Hung-Chih Chen
    • Giou-Teng Yiang
  • View Affiliations

  • Published online on: February 1, 2010     https://doi.org/10.3892/ijo_00000508
  • Pages: 365-370
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Abstract

Recombinant adenovirus-associated vector serotype 2 (rAAV2) is one of the most promising gene transfer vectors due to its advantage of causing non-pathogenic infection, low immunogenicity, and long-term gene expression in human clinical trials. Human interleukin 15 (hIL15) has been implicated in modulation of antitumor activity of lymphokine-activated killer (LAK) cells, including T cells and NK cells. In this study, the rAAV2-hIL15 vector was produced and subjected for treatment with xenograft JC breast cancer model. Results showed that tumor onset was significantly delayed, the tumor growth was suppressed, and the lifespan of tumor-bearing mice were prolonged by rAAV2-hIL15. In addition, rAAV2-hIL15 was able to produce a substantial expression of IL15 protein that ultimately activated the cytotoxic activity of LAK cells. Furthermore, prominent apoptosis was observed in tumor lesions following injection of rAAV2-hIL15. Taken together, our results suggested that rAAV2-hIL15 appears as a new potential therapeutic tool for breast cancer immunotherapy.

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February 2010
Volume 36 Issue 2

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Yu Y, Wei C, Chen Y, Chen MH and Yiang G: Immunotherapy of breast cancer by single delivery with rAAV2-mediated interleukin-15 expression. Int J Oncol 36: 365-370, 2010.
APA
Yu, Y., Wei, C., Chen, Y., Chen, M.H., & Yiang, G. (2010). Immunotherapy of breast cancer by single delivery with rAAV2-mediated interleukin-15 expression. International Journal of Oncology, 36, 365-370. https://doi.org/10.3892/ijo_00000508
MLA
Yu, Y., Wei, C., Chen, Y., Chen, M. H., Yiang, G."Immunotherapy of breast cancer by single delivery with rAAV2-mediated interleukin-15 expression". International Journal of Oncology 36.2 (2010): 365-370.
Chicago
Yu, Y., Wei, C., Chen, Y., Chen, M. H., Yiang, G."Immunotherapy of breast cancer by single delivery with rAAV2-mediated interleukin-15 expression". International Journal of Oncology 36, no. 2 (2010): 365-370. https://doi.org/10.3892/ijo_00000508