Knockdown of STAT3 expression by RNAi suppresses growth and induces apoptosis and differentiation in glioblastoma stem cells

  • Authors:
    • Guang-Hui Li
    • Hong Wei
    • Sheng-Qing Lv
    • Hua Ji
    • Dong-Lin Wang
  • View Affiliations

  • Published online on: July 1, 2010     https://doi.org/10.3892/ijo_00000658
  • Pages: 103-110
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Abstract

Glioblastoma is a highly lethal brain tumor of the human primary nervous system tumors. Previous studies demonstrated that glioblastoma stem cells were able to initiate and reform the original cancer. In this study, we found that there were expression and activation of STAT3, a key signal transduction factor and oncoprotein, in human glioblastoma stem cells (GSCs). STAT3 plays a key role in proliferation, apoptosis and differentiation in embryonic stem cells and several cancer types. To investigate the effects of STAT3 on human GSCs, the expression and activation of STAT3 were suppressed by RNAi mediated with lentivirus. We demonstrated that siRNA of STAT3 significantly suppressed STAT3 expression and activation and resulted in inhibition of cell growth in GSCs. Knockdown of STAT3 induces apoptosis and reduces significantly expression of Bcl-2 and cyclin-D in human primary GSCs, whereas no significance was achieved in BAX and caspase-3 expression. Inhibition of STAT3 expression is associated not only with decreasing of CD133+ cell proportion and increasing of GFAP and MBP exression, but also with decrease of the capacity to initiate a tumor in human primary GSCs. Together, these studies suggest that STAT3 is an important target for human GSCs in regulation of GSCs growth, apoptosis, differentiation and tumorigenic potential.

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July 2010
Volume 37 Issue 1

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Li G, Wei H, Lv S, Ji H and Wang D: Knockdown of STAT3 expression by RNAi suppresses growth and induces apoptosis and differentiation in glioblastoma stem cells. Int J Oncol 37: 103-110, 2010.
APA
Li, G., Wei, H., Lv, S., Ji, H., & Wang, D. (2010). Knockdown of STAT3 expression by RNAi suppresses growth and induces apoptosis and differentiation in glioblastoma stem cells. International Journal of Oncology, 37, 103-110. https://doi.org/10.3892/ijo_00000658
MLA
Li, G., Wei, H., Lv, S., Ji, H., Wang, D."Knockdown of STAT3 expression by RNAi suppresses growth and induces apoptosis and differentiation in glioblastoma stem cells". International Journal of Oncology 37.1 (2010): 103-110.
Chicago
Li, G., Wei, H., Lv, S., Ji, H., Wang, D."Knockdown of STAT3 expression by RNAi suppresses growth and induces apoptosis and differentiation in glioblastoma stem cells". International Journal of Oncology 37, no. 1 (2010): 103-110. https://doi.org/10.3892/ijo_00000658