Open Access

Sorafenib induces apoptosis and autophagy in prostate cancer cells in vitro

  • Authors:
    • Anders Ullén
    • Marianne Farnebo
    • Lena Thyrell
    • Salah Mahmoudi
    • Pedram Kharaziha
    • Lena Lennartsson
    • Dan Grandér
    • Theoharis Panaretakis
    • Sten Nilsson
  • View Affiliations

  • Published online on: Thursday, July 1, 2010
  • Pages:15-20 DOI: 10.3892/ijo_00000648
0

Abstract

The multiple tyrosine kinase inhibitor sorafenib has recently demonstrated clinical effects in patients with androgen-independent prostate cancer. These observations provided the rational for investigating the anti-tumoral properties of this compound on prostate cancer cell lines at the molecular level. Two hormone refractory (PC3 and DU145) and one hormone responsive cell line (22Rv1) were used. By use of a panel of cell biology techniques such as immunoblotting, flow cytometry and immunocytochemistry, effects on the MAPK pathway and induction of apoptosis and autophagy were evaluated. We demonstrate that sorafenib reduced cell viability in a dose-dependent manner, induced apoptosis and inactivated the MAPK pathway. Moreover, we show for the first time, that sorafenib treatment of prostate cancer cells also induces cellular autophagy. This feature is in accordance with the anticancer potential of sorafenib and adds another important effector mechanism of this compound. These observations may open potentially interesting treatment combinations that may augment the effect of sorafenib, either by drugs that promote autophagy such as the rapalogues, or by combining sorafenib with compounds that specifically inhibit the autophagic process.

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July 2010
Volume 37 Issue 1

Print ISSN: 1019-6439
Online ISSN:1791-2423

2015 Impact Factor: 3.018
Ranked #30/211 Oncology
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APA
Ullén, A., Farnebo, M., Thyrell, L., Mahmoudi, S., Kharaziha, P., Lennartsson, L. ... Nilsson, S. (2010). Sorafenib induces apoptosis and autophagy in prostate cancer cells in vitro. International Journal of Oncology, 37, 15-20. http://dx.doi.org/10.3892/ijo_00000648
MLA
Ullén, A., Farnebo, M., Thyrell, L., Mahmoudi, S., Kharaziha, P., Lennartsson, L., Grandér, D., Panaretakis, T., Nilsson, S."Sorafenib induces apoptosis and autophagy in prostate cancer cells in vitro". International Journal of Oncology 37.1 (2010): 15-20.
Chicago
Ullén, A., Farnebo, M., Thyrell, L., Mahmoudi, S., Kharaziha, P., Lennartsson, L., Grandér, D., Panaretakis, T., Nilsson, S."Sorafenib induces apoptosis and autophagy in prostate cancer cells in vitro". International Journal of Oncology 37, no. 1 (2010): 15-20. http://dx.doi.org/10.3892/ijo_00000648