Effect of age on the association between p16CDKN2A methylation and DNMT3B polymorphism in head and neck carcinoma and patient survival

  • Authors:
    • Lucyana Conceição Farias
    • Carlos Alberto De Carvalho Fraga
    • Marcos Vinícius Macedo De Oliveira
    • Thiago Fonseca Silva
    • Luciano Marques-Silva
    • Paula Rocha Moreira
    • Alfredo Maurício Batista De-Paula
    • Ricardo Santiago Gomez
    • André Luiz Sena Guimarães
  • View Affiliations

  • Published online on: July 1, 2010     https://doi.org/10.3892/ijo_00000664
  • Pages: 167-176
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Abstract

De novo DNA methylation is a relevant epigenetic mechanism, which represses gene transcription and commonly inactivates tumor suppressor genes in carcinogenesis. A single nucleotide polymorphism of DNMT3B, C46359T (−149C↷T) was reported to modulate individual's susceptibility to cancer. We investigated the role of this polymorphic variant regarding the methylation status of the p16CDKN2A gene in young and older patients with head and neck squamous cell carcinoma (HNCC) matched by the TNM staging system, together with its impact on patients survival. The results showed that the presence of the allele T of the polymorphism DNMT3B (−149C↷T) was associated with advanced TNM staging and smoking habit, but no association was found between this polymorphisms and DNMT3B immunostaining. While p16CDKN2A methylation was significantly associated with smoking habit in older patients, this parameter was associated with family history of cancer in young patients. Moreover, in older patients the absence of p16CDKN2A promoter methylation had a negative impact on survival. In conclusion, nucleotide polymorphism of DNMT3B is not associated with methylation of p16CDKN2A gene in HNSCC. The association of p16CDKN2A gene methylation with smoking, family history of cancer and survival is dependent on age.

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July 2010
Volume 37 Issue 1

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Farias LC, De Carvalho Fraga CA, De Oliveira MV, Silva TF, Marques-Silva L, Moreira PR, De-Paula AM, Gomez RS and Guimarães AL: Effect of age on the association between p16CDKN2A methylation and DNMT3B polymorphism in head and neck carcinoma and patient survival. Int J Oncol 37: 167-176, 2010
APA
Farias, L.C., De Carvalho Fraga, C.A., De Oliveira, M.V., Silva, T.F., Marques-Silva, L., Moreira, P.R. ... Guimarães, A.L. (2010). Effect of age on the association between p16CDKN2A methylation and DNMT3B polymorphism in head and neck carcinoma and patient survival. International Journal of Oncology, 37, 167-176. https://doi.org/10.3892/ijo_00000664
MLA
Farias, L. C., De Carvalho Fraga, C. A., De Oliveira, M. V., Silva, T. F., Marques-Silva, L., Moreira, P. R., De-Paula, A. M., Gomez, R. S., Guimarães, A. L."Effect of age on the association between p16CDKN2A methylation and DNMT3B polymorphism in head and neck carcinoma and patient survival". International Journal of Oncology 37.1 (2010): 167-176.
Chicago
Farias, L. C., De Carvalho Fraga, C. A., De Oliveira, M. V., Silva, T. F., Marques-Silva, L., Moreira, P. R., De-Paula, A. M., Gomez, R. S., Guimarães, A. L."Effect of age on the association between p16CDKN2A methylation and DNMT3B polymorphism in head and neck carcinoma and patient survival". International Journal of Oncology 37, no. 1 (2010): 167-176. https://doi.org/10.3892/ijo_00000664